Cytotoxic Activity and Cytokine Production by CTL Clones
| Clone . | Percent Lysis* . | Cytokine Production3-151 . | ||
|---|---|---|---|---|
| BALB/c . | bm1 . | IFN-γ (ng/mL) . | TNF-α (ng/mL) . | |
| 14C3 | 623-152 | 1 | 2,500 | 4.8 |
| 3F3 | 28 | 1 | 18,750 | 45.0 |
| 7C11 | 56 | −1 | 6,500 | 10.6 |
| Clone . | Percent Lysis* . | Cytokine Production3-151 . | ||
|---|---|---|---|---|
| BALB/c . | bm1 . | IFN-γ (ng/mL) . | TNF-α (ng/mL) . | |
| 14C3 | 623-152 | 1 | 2,500 | 4.8 |
| 3F3 | 28 | 1 | 18,750 | 45.0 |
| 7C11 | 56 | −1 | 6,500 | 10.6 |
*Percent lysis was tested in a 4-hour 51Cr release assay with ConA blasts of the indicated strains. Results are shown at a 25:1 effector to target ratio.
Cytokine production was determined with supernatants from 2.0-mL cultures containing 1.0 × 106 cells in wells coated with CD3-specific antibody 145-2C11 (10 μg/mL in pH 9.4 buffer for 24 hours). Samples collected after 24 hours were stored at −20°C until testing.
The cytotoxic specificity of clone 14C3 was mapped to H2Kd by testing B10.D2 (R107) and B10.D2 (R103) targets.