Cell Surface Expression of CD4, HLA-DR, HLA-DP, and HLA-DQ Determinations on RAJI-CD4 and RM3-CD4 Cells
| Antibody (clone) . | RAJI-CD4 . | RM3-CD4 . | ||
|---|---|---|---|---|
| . | % Positive Cells . | MFI . | % Positive Cells . | MFI . |
| Anti-CD4 (Leu-3A) | 73.6 | 75.7 | 53.6 | 64.6 |
| Anti–HLA-DR (2.06) | 99.0 | 130.7 | 0.8 | 12.3 |
| Anti–HLA-DP (B7.21) | 98.5 | 95.5 | 0.6 | 11.1 |
| Anti–HLA-DQ (BT3.4) | 98.1 | 92.9 | 0.9 | 13.2 |
| Antibody (clone) . | RAJI-CD4 . | RM3-CD4 . | ||
|---|---|---|---|---|
| . | % Positive Cells . | MFI . | % Positive Cells . | MFI . |
| Anti-CD4 (Leu-3A) | 73.6 | 75.7 | 53.6 | 64.6 |
| Anti–HLA-DR (2.06) | 99.0 | 130.7 | 0.8 | 12.3 |
| Anti–HLA-DP (B7.21) | 98.5 | 95.5 | 0.6 | 11.1 |
| Anti–HLA-DQ (BT3.4) | 98.1 | 92.9 | 0.9 | 13.2 |
Cells were analyzed by flow cytometry after staining with the indicated MoAbs in conjunction with a fluorescein-labeled goat antimouse antibody.
Abbreviation: MFI, mean fluorescence intensity.