Expression of Myeloid Cell Surface Markers on NB4 and in NB4-AsR Cells (% positive cells)
| . | Medium . | RA 10−6 mol/L . | As 10−8 mol/L . | As 10−7 mol/L . | RA + As 10−8 mol/L . | RA + As 10−7 mol/L . |
|---|---|---|---|---|---|---|
| NB4 | ||||||
| CD33 | 86.5 | 60 | 80 | 60 | 30 | 38 |
| CD11b | 13.8 | 76 | 36 | 73 | 68 | 65 |
| CD11c | 0 | 80 | 53 | 80 | 80 | 81.5 |
| CD14 | 0 | 34 | 40 | 49 | 0 | 0 |
| NB4-AsR | ||||||
| CD33 | 93 | 60 | 85 | 65 | 0 | 0 |
| CD11b | 25 | 76 | 92 | 74 | 69 | 70 |
| CD11c | 25 | 80 | 95 | 86 | 74 | 70 |
| CD14 | 56 | 60 | 50 | 38 | 0 | 0 |
| . | Medium . | RA 10−6 mol/L . | As 10−8 mol/L . | As 10−7 mol/L . | RA + As 10−8 mol/L . | RA + As 10−7 mol/L . |
|---|---|---|---|---|---|---|
| NB4 | ||||||
| CD33 | 86.5 | 60 | 80 | 60 | 30 | 38 |
| CD11b | 13.8 | 76 | 36 | 73 | 68 | 65 |
| CD11c | 0 | 80 | 53 | 80 | 80 | 81.5 |
| CD14 | 0 | 34 | 40 | 49 | 0 | 0 |
| NB4-AsR | ||||||
| CD33 | 93 | 60 | 85 | 65 | 0 | 0 |
| CD11b | 25 | 76 | 92 | 74 | 69 | 70 |
| CD11c | 25 | 80 | 95 | 86 | 74 | 70 |
| CD14 | 56 | 60 | 50 | 38 | 0 | 0 |
Effect of As2O3, RA or the combination of the two compounds on the expression of the myeloid surface markers CD33, CD11b, CD11c, and CD14. NB4 cells and NB4-AsR cells (105/mL) were incubated for 3 days with the different compounds. The number of positive cells for the expression of the various markers, as well as the mean associated fluorescence, were quantified by flow cytometry. One presentative experiment among 3 different assays is reported.