Table 1.

Purification of rhCDD Expressed in E coli

StepTotal Protein (mg) Total Activity (U × 10−3) Specific Activity (U × 10−3/mg) Purification (fold)Activity Recovery (%)
E coli extract  225 1,720  7.6  1  100  
Affi-Gel Blue  35  1,590 45.4  6  92  
Mono Q  7  820  117.0  15  48 
Mono S  4  540  135.0  18  31 
StepTotal Protein (mg) Total Activity (U × 10−3) Specific Activity (U × 10−3/mg) Purification (fold)Activity Recovery (%)
E coli extract  225 1,720  7.6  1  100  
Affi-Gel Blue  35  1,590 45.4  6  92  
Mono Q  7  820  117.0  15  48 
Mono S  4  540  135.0  18  31 
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