Colony Formation From CD34+EGFR+ and CD34+ EGFR− Cells in Clonal Culture
| . | No. of Colonies per 200 Cells . | ||||
|---|---|---|---|---|---|
| CFU-Mix . | BFU-E . | CFU-GM . | CFU-M . | Total . | |
| CD34+ EGFR+ | 2.3 ± 1.2 | 9.3 ± 1.5 | 43.0 ± 5.2 | 5.7 ± 0.6 | 60.3 ± 6.0 |
| CD34+ EGFR− | 1.0 ± 0.0 | 4.7 ± 2.5 | 53.3 ± 5.9 | 6.7 ± 1.2 | 65.7 ± 7.2 |
| . | No. of Colonies per 200 Cells . | ||||
|---|---|---|---|---|---|
| CFU-Mix . | BFU-E . | CFU-GM . | CFU-M . | Total . | |
| CD34+ EGFR+ | 2.3 ± 1.2 | 9.3 ± 1.5 | 43.0 ± 5.2 | 5.7 ± 0.6 | 60.3 ± 6.0 |
| CD34+ EGFR− | 1.0 ± 0.0 | 4.7 ± 2.5 | 53.3 ± 5.9 | 6.7 ± 1.2 | 65.7 ± 7.2 |
Enriched CD34+ cells were infected with the Ax/hEGFR virus and sorted into CD34+ EGFR+ versus CD34+ EGFR− cell populations. Two hundred cells of each population were cultured in methylcellulose culture with SCF, IL-6, IL-3, G-CSF, Tpo, and Epo, and colonies were scored on day 14. Results represent the mean number of colonies per 200 cells ± SD of triplicate cultures.