Effect of EGF Through Adenovirus-Mediated EGFR on the Expansion of Colony-Forming Cells
| Virus . | EGF . | No. of Colonies Derived From 200 CD34+ Cells . | |
|---|---|---|---|
| CFU-GM . | BFU-E . | ||
| (−) | (−) (+) | 29.4 ± 2.6 26.0 ± 9.3 | 2.6 ± 1.2 2.6 ± 1.5 |
| Ax1w | (−) (+) | 35.1 ± 11.5 38.6 ± 8.0 | 2.4 ± 1.5 2.8 ± 1.4 |
| Ax/hEGFR | (−) (+) | 36.4 ± 10.0 107.5 ± 38.7 | 2.6 ± 1.0 38.2 ± 27.5 |
| Virus . | EGF . | No. of Colonies Derived From 200 CD34+ Cells . | |
|---|---|---|---|
| CFU-GM . | BFU-E . | ||
| (−) | (−) (+) | 29.4 ± 2.6 26.0 ± 9.3 | 2.6 ± 1.2 2.6 ± 1.5 |
| Ax1w | (−) (+) | 35.1 ± 11.5 38.6 ± 8.0 | 2.4 ± 1.5 2.8 ± 1.4 |
| Ax/hEGFR | (−) (+) | 36.4 ± 10.0 107.5 ± 38.7 | 2.6 ± 1.0 38.2 ± 27.5 |
One-milliliter cultures were initiated with 1.0 × 104 enriched CD34+ cells in a medium containing IL-6 and SCF in the presence or absence of EGF. These cells were incubated with the Ax1w or Ax/hEGFR virus, and control cells were cultured without adenovirus vectors. After 5 days of incubation, 1/50 of the cells of each fraction were subjected to methylcellulose clonal culture with SCF, IL-6, IL-3, and Epo in duplicate. The experiments were repeated five times using CD34+ cells from 5 patients, respectively. The mean ± SD of five experiments is shown.