The VL and JL Germline Segments Used by Amyloidogenic Light Chains and the Number of Identical/Sequenced Plasmid Inserts
| Amyloid Clones . | VL Germline (family) . | Nucleotide Identity* (%) . | CDR . | FR . | Identical/ Sequenced Inserts . | ||||
|---|---|---|---|---|---|---|---|---|---|
| R . | S . | P (CDR)† . | R . | S . | P (FR) . | ||||
| QUA BJκ | B3 (κIV) | 94.1 | 9 (4.4)‡ | 3 | .012 | 3 (9.4) | 3 | .002 | 9/9 |
| TAU BJκ | O2/O12 (κI) | 91.6 | 9 (5.0) | 3 | .029 | 6 (13.4) | 6 | .002 | 8/9 |
| IAC BJκ | L2 (κIII) | 91.9 | 4 (4.7) | 0 | .202 | 13 (12.8) | 6 | .165 | 8/8 |
| ORR BJκ | L1 (κI) | 94.4 | 4 (3.3) | 1 | .205 | 7 (8.9) | 4 | .125 | 7/7 |
| DIB BJλ | λIII.1 (λIII) | 94.0 | 9 (3.6) | 2 | .003 | 5 (9.2) | 1 | .025 | 7/7 |
| PAP BJλ | λIII.1 (λII) | 89.8 | 15 (6.1) | 2 | .0002 | 8 (15.7) | 4 | .002 | 5/6 |
| SEM BJλ | λIII.1 (λIII) | 94.7 | 6 (3.2) | 2 | .053 | 5 (8.1) | 2 | .057 | 6/6 |
| FER BJλ | 1b (λI) | 95.9 | 7 (2.8) | 1 | .008 | 2 (6.1) | 2 | .014 | 5/5 |
| GHE BJλ | 2b2 (λIII) | 93.3 | 6 (4.5) | 2 | .144 | 8 (10.4) | 4 | .1 | 6/6 |
| FIL BJλ | 4b (λIV) | 96.0 | 7 (2.8) | 1 | .008 | 2 (6.3) | 2 | .01 | 5/6 |
| CAR IgGκ | O8/O18 (κI) | 95.8 | 5 (2.5) | 1 | .063 | 4 (6.7) | 2 | .070 | 9/10 |
| SET IgGλ | λ3.1 (λIII) | 95.4 | 7 (2.7) | 1 | .008 | 3 (7.0) | 2 | .019 | 3/10 |
| MAR IgAλ | 2b2 (λII) | 92.6 | 7 (5.0) | 0 | .111 | 10 (11.4) | 5 | .140 | 7/11 |
| DEP IgAλ | λIII.1 (λIII) | 95.4 | 5 (2.8) | 2 | .082 | 4 (7.0) | 2 | .055 | 9/9 |
| Amyloid Clones . | VL Germline (family) . | Nucleotide Identity* (%) . | CDR . | FR . | Identical/ Sequenced Inserts . | ||||
|---|---|---|---|---|---|---|---|---|---|
| R . | S . | P (CDR)† . | R . | S . | P (FR) . | ||||
| QUA BJκ | B3 (κIV) | 94.1 | 9 (4.4)‡ | 3 | .012 | 3 (9.4) | 3 | .002 | 9/9 |
| TAU BJκ | O2/O12 (κI) | 91.6 | 9 (5.0) | 3 | .029 | 6 (13.4) | 6 | .002 | 8/9 |
| IAC BJκ | L2 (κIII) | 91.9 | 4 (4.7) | 0 | .202 | 13 (12.8) | 6 | .165 | 8/8 |
| ORR BJκ | L1 (κI) | 94.4 | 4 (3.3) | 1 | .205 | 7 (8.9) | 4 | .125 | 7/7 |
| DIB BJλ | λIII.1 (λIII) | 94.0 | 9 (3.6) | 2 | .003 | 5 (9.2) | 1 | .025 | 7/7 |
| PAP BJλ | λIII.1 (λII) | 89.8 | 15 (6.1) | 2 | .0002 | 8 (15.7) | 4 | .002 | 5/6 |
| SEM BJλ | λIII.1 (λIII) | 94.7 | 6 (3.2) | 2 | .053 | 5 (8.1) | 2 | .057 | 6/6 |
| FER BJλ | 1b (λI) | 95.9 | 7 (2.8) | 1 | .008 | 2 (6.1) | 2 | .014 | 5/5 |
| GHE BJλ | 2b2 (λIII) | 93.3 | 6 (4.5) | 2 | .144 | 8 (10.4) | 4 | .1 | 6/6 |
| FIL BJλ | 4b (λIV) | 96.0 | 7 (2.8) | 1 | .008 | 2 (6.3) | 2 | .01 | 5/6 |
| CAR IgGκ | O8/O18 (κI) | 95.8 | 5 (2.5) | 1 | .063 | 4 (6.7) | 2 | .070 | 9/10 |
| SET IgGλ | λ3.1 (λIII) | 95.4 | 7 (2.7) | 1 | .008 | 3 (7.0) | 2 | .019 | 3/10 |
| MAR IgAλ | 2b2 (λII) | 92.6 | 7 (5.0) | 0 | .111 | 10 (11.4) | 5 | .140 | 7/11 |
| DEP IgAλ | λIII.1 (λIII) | 95.4 | 5 (2.8) | 2 | .082 | 4 (7.0) | 2 | .055 | 9/9 |
*When compared with the closest germline gene. The leader is excluded from identity calculations to allow comparison with other studies.
Probability that the observed R mutations occurred by chance (calculations according to Chang and Casali11).
Number in parentheses indicates number of R mutations expected by chance.