The VH and JH Germline Segments Used by Three Amyloidogenic Clones and the Number of Identical/Sequenced Plasmid Inserts
| Amyloid Clones . | VH Germline (family) . | Nucleotide Identity* (%) . | CDR . | FR . | Identical/ Sequenced Inserts . | ||||
|---|---|---|---|---|---|---|---|---|---|
| R . | S . | P (CDR)† . | R . | S . | P (FR) . | ||||
| CAR IgGκ | 3-48 (VHIII) | 92.2 | 3 (4.2)‡ | 1 | .193 | 12 (13.2) | 7 | .144 | 6/7 |
| MAR IgAλ | 3-30 (VHIII) | 89.5 | 8 (5.4) | 3 | .082 | 12 (17.9) | 8 | .015 | 6/8 |
| DEP IgAλ | 1-18 (VHI) | 96.3 | 5 (1.9) | 0 | .025 | 5 (6.4) | 1 | .165 | 6/6 |
| Amyloid Clones . | VH Germline (family) . | Nucleotide Identity* (%) . | CDR . | FR . | Identical/ Sequenced Inserts . | ||||
|---|---|---|---|---|---|---|---|---|---|
| R . | S . | P (CDR)† . | R . | S . | P (FR) . | ||||
| CAR IgGκ | 3-48 (VHIII) | 92.2 | 3 (4.2)‡ | 1 | .193 | 12 (13.2) | 7 | .144 | 6/7 |
| MAR IgAλ | 3-30 (VHIII) | 89.5 | 8 (5.4) | 3 | .082 | 12 (17.9) | 8 | .015 | 6/8 |
| DEP IgAλ | 1-18 (VHI) | 96.3 | 5 (1.9) | 0 | .025 | 5 (6.4) | 1 | .165 | 6/6 |
*When compared with the closest germline gene. The leader is excluded from identity calculations to allow comparison with other studies.
Probability that the observed R mutations occurred by chance (calculations according to Chang and Casali11).
Number in parentheses indicates number of R mutations expected by chance.