Viral Detection in PBMC of Rabbits Inoculated With Transfected Cells
| Transfectant . | Rabbit (no. inoc.)-150 . | Test-151 . | No. Rabbits Positive/No. Monitored at Indicated Week Postinoculation . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 . | 1 . | 2 . | 4 . | 6 . | 8 . | 10 . | 12 . | 16 . | 20 . | 26 . | |||
| ACH | 31, 32 (2) | p19 | 0/2 | ND | 0/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 |
| PCR | 0/4 | ND | 0/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 1/2 | 2/2 | 2/2 | ||
| 51-54 (1) | p19 | 0/4 | 1/4 | 2/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | —-153 | — | — | |
| PCR | 0/4 | 1/4-152 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | — | — | — | ||
| ACH.p12 | 33, 34 (2) | p19 | 0/2 | ND | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| PCR | 0/6 | ND | 0/2 | 0/2 | 2/2-152 | 1/2-152 | 2/2-152 | 2/2-152 | 1/2-152 | 0/2 | 0/2 | ||
| 55-58 (1) | p19 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | — | — | — | |
| PCR | 0/4 | 0/4 | 2/4-152 | 1/4-152 | 0/4 | 0/4 | 0/4 | 0/4 | — | — | — | ||
| pKS | 50 (1) | p19 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | — | — | — |
| PCR | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | — | — | — | ||
| Transfectant . | Rabbit (no. inoc.)-150 . | Test-151 . | No. Rabbits Positive/No. Monitored at Indicated Week Postinoculation . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 . | 1 . | 2 . | 4 . | 6 . | 8 . | 10 . | 12 . | 16 . | 20 . | 26 . | |||
| ACH | 31, 32 (2) | p19 | 0/2 | ND | 0/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 |
| PCR | 0/4 | ND | 0/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 1/2 | 2/2 | 2/2 | ||
| 51-54 (1) | p19 | 0/4 | 1/4 | 2/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | —-153 | — | — | |
| PCR | 0/4 | 1/4-152 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | — | — | — | ||
| ACH.p12 | 33, 34 (2) | p19 | 0/2 | ND | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| PCR | 0/6 | ND | 0/2 | 0/2 | 2/2-152 | 1/2-152 | 2/2-152 | 2/2-152 | 1/2-152 | 0/2 | 0/2 | ||
| 55-58 (1) | p19 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | — | — | — | |
| PCR | 0/4 | 0/4 | 2/4-152 | 1/4-152 | 0/4 | 0/4 | 0/4 | 0/4 | — | — | — | ||
| pKS | 50 (1) | p19 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | — | — | — |
| PCR | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | — | — | — | ||
Abbreviation: ND, not determined.
Rabbits were inoculated once (R50-58) or twice (R31-34) with indicated transfectant.
p19, ELISA measurement of p19 antigen in 7-day supernatants of ex vivo PBMC cultures, positive indicates concentrations of at least 15 pg/mL; PCR, amplification of HTLV-1 ORF I/II–specific proviral sequence from rabbit PBMC. PCR sensitivity was estimated to be 1 proviral genome per 5,000 cells.
Faint positives.
Rabbits 50-58 were killed at 12 weeks (termination of study).