Effect of Ragweed Challenge on Leukocyte Rolling and Adhesion in Mesenteric Venules
| Leukocyte Rolling per Minute (mean ± SE) . | Leukocyte Adhesion (adherent leukocytes/100 μm venule length; mean ± SE) . | ||||||
|---|---|---|---|---|---|---|---|
| Mice . | Ragweed Challenged . | Diluent Challenged . | P . | Mice . | Ragweed Challenged . | Diluent Challenged . | P . |
| Wild-type | 68.1 ± 11.1 | 9.1 ± 1.5 | .004 | Wild-type | 10.6 ± 2.2 | 2.3 ± 0.9 | .02 |
| P-selectin–deficient | 4.2 ± 0.3 | 0-150 | NS | P-selectin–deficient | 2.8 ± 1.2 | 0.2 ± 0.4 | .04 |
| ICAM-1–deficient | 45.1 ± 4.5 | 13.9 ± 2.2 | .002 | ICAM-1–deficient | 1.1 ± 0.4 | 0.6 ± 0.3 | NS |
| Leukocyte Rolling per Minute (mean ± SE) . | Leukocyte Adhesion (adherent leukocytes/100 μm venule length; mean ± SE) . | ||||||
|---|---|---|---|---|---|---|---|
| Mice . | Ragweed Challenged . | Diluent Challenged . | P . | Mice . | Ragweed Challenged . | Diluent Challenged . | P . |
| Wild-type | 68.1 ± 11.1 | 9.1 ± 1.5 | .004 | Wild-type | 10.6 ± 2.2 | 2.3 ± 0.9 | .02 |
| P-selectin–deficient | 4.2 ± 0.3 | 0-150 | NS | P-selectin–deficient | 2.8 ± 1.2 | 0.2 ± 0.4 | .04 |
| ICAM-1–deficient | 45.1 ± 4.5 | 13.9 ± 2.2 | .002 | ICAM-1–deficient | 1.1 ± 0.4 | 0.6 ± 0.3 | NS |
Values are the mean ± SE of 5 to 6 mice per experimental group. In each mouse, a total of 3 to 10 postcapillary venules were analyzed. The number of rolling leukocytes was quantitated by counting the leukocytes passing through a perpendicular plane during a 1-minute interval. Firm adhesion of leukocytes is expressed as the number of adherent cells/100 μm of venular length.
Abbreviation: NS, not significant.
No leukocyte rolling was detected during the 1-hour observation period.