Hemodynamic Parameters of the Postcapillary Venules of Allergen- and Saline-Challenged Mice
| Hemodynamic Parameters . | Wild-Type . | P-Selectin–Deficient . | ICAM-1–Deficient . | |||
|---|---|---|---|---|---|---|
| Ragweed . | Saline . | Ragweed . | Saline . | Ragweed . | Saline . | |
| Venular diameter (μm) | 48 ± 4 | 47 ± 9 | 38 ± 5 | 40 ± 3 | 46 ± 3 | 44 ± 5 |
| Vblood* (mm/s) | 2.6 ± 0.6 | 2.1 ± 0.5 | 1.5 ± 0.3 | 1.8 ± 0.2 | 1.2 ± 0.1 | 1.7 ± 0.3 |
| Shear rate (s−1) | 248 ± 23 | 196 ± 11.4 | 170 ± 31 | 260 ± 34 | 173 ± 14 | 330 ± 47 |
| Shear stress (dyn/cm1) | 6.2 ± 0.6 | 4.9 ± 0.3 | 4.2 ± 0.8 | 6.5 ± 0.9 | 4.2 ± 0.3 | 8.3 ± 1.2 |
| Hemodynamic Parameters . | Wild-Type . | P-Selectin–Deficient . | ICAM-1–Deficient . | |||
|---|---|---|---|---|---|---|
| Ragweed . | Saline . | Ragweed . | Saline . | Ragweed . | Saline . | |
| Venular diameter (μm) | 48 ± 4 | 47 ± 9 | 38 ± 5 | 40 ± 3 | 46 ± 3 | 44 ± 5 |
| Vblood* (mm/s) | 2.6 ± 0.6 | 2.1 ± 0.5 | 1.5 ± 0.3 | 1.8 ± 0.2 | 1.2 ± 0.1 | 1.7 ± 0.3 |
| Shear rate (s−1) | 248 ± 23 | 196 ± 11.4 | 170 ± 31 | 260 ± 34 | 173 ± 14 | 330 ± 47 |
| Shear stress (dyn/cm1) | 6.2 ± 0.6 | 4.9 ± 0.3 | 4.2 ± 0.8 | 6.5 ± 0.9 | 4.2 ± 0.3 | 8.3 ± 1.2 |
There was no statistically significant difference in hemodynamic parameters obtained between the various groups (ragweed vsaline; P-selectin or ICAM-1 v wild-type).
Mean blood flow velocity (millimeters per second) was calculated from the highest eosinophil velocity in each of the different venules assuming a parabolic flow. The wall shear stress was determined from the shear rate assuming blood viscosity of 0.025 poise.