Effect of Ragweed Challenge on Eosinophil Rolling and Adhesion to Endothelium in Mesenteric Venules of Wild-Type and P-Selectin–Deficient Mice
| Eosinophil Rolling Fraction (%; mean ± SE) . | Eosinophil Adhesion (adherent eosinophils/100 μm venule length; mean ± SE) . | ||||||
|---|---|---|---|---|---|---|---|
| Mice . | Ragweed Challenged . | Diluent Challenged . | P . | Mice . | Ragweed Challenged . | Diluent Challenged . | P . |
| Wild-type | 26.7 ± 2.6 | 9.1 ± 1.5 | .002 | Wild-type | 3.3 ± 1.1 | 0.4 ± 0.3 | .001 |
| P-selectin–deficient | 1.2 ± 0.8 | 0* | NS | P-selectin–deficient | 0.8 ± 0.5 | 0* | .03 |
| Eosinophil Rolling Fraction (%; mean ± SE) . | Eosinophil Adhesion (adherent eosinophils/100 μm venule length; mean ± SE) . | ||||||
|---|---|---|---|---|---|---|---|
| Mice . | Ragweed Challenged . | Diluent Challenged . | P . | Mice . | Ragweed Challenged . | Diluent Challenged . | P . |
| Wild-type | 26.7 ± 2.6 | 9.1 ± 1.5 | .002 | Wild-type | 3.3 ± 1.1 | 0.4 ± 0.3 | .001 |
| P-selectin–deficient | 1.2 ± 0.8 | 0* | NS | P-selectin–deficient | 0.8 ± 0.5 | 0* | .03 |
Values are mean ± SE of 5 mice per experimental group. In each group, 2 to 5 postcapillary venules were investigated. The rolling of injected eosinophils (fluorescently labeled) in mesenteric venules are expressed as a fraction of total injected cells (Rf, %). Firm adhesion of injected eosinophils (fluorescently labeled) are expressed as the number of adherent eosinophils/100 μm of venule length.
Abbreviation: NS, not significant.
No eosinophil rolling or adhesion was detected during the 1-hour observation period.