Effects of PEG-rHuMGDF on Platelet Production in Thrombocytopenic HIV-Infected Chimpanzees
| Determination . | Normal Controls . | HIV-Thrombocytopenic Chimpanzees . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mean Values . | No. 1 . | No. 2 . | No. 3 . | ||||||
| Before Therapy . | 10 d After Therapy . | Before . | After . | Before . | After . | Before . | After . | ||
| Platelets | |||||||||
| Concentration (platelets/μL) | 228 ± 92 | 64 ± 19 | 599 ± 260 | 52 | 547 | 86 | 881 | 60 | 368 |
| MPV (fL) | 10.9 ± 0.7 | 11.2 ± 1.8 | 9.5 ± 0.9 | 12.1 | 10.6 | 9.1 | 8.9 | 12.3 | 9.1 |
| Antiplatelet GPIIIa49-66 ab (×10−3 units) | <20 | 333 ± 278 | 169 ± 164 | 648 | 339 | 123 | 11 | 229 | 157 |
| Megakaryocytes | |||||||||
| No. (×106/kg) | 11 ± 2.1 | 11.7 ± 6.5 | 353 ± 255 | 11 | 182 | 19 | 647 | 5.8 | 231 |
| Volume (×103/fL) | 28.0 ± 4.5 | 38.1 ± 1.9 | 18.7 ± 2.1 | 37 | 20 | 40 | 16 | 37 | 20 |
| Mass (×1010 fL/kg) | 31 ± 5.3 | 45.2 ± 27.2 | 624 ± 373 | 39 | 357 | 75 | 1,050 | 21.4 | 464 |
| Progenitors/103 CD34+ | 3.6 ± 0.6 | 14.1 | 4.4 | 3.2 | 3.2 | 14.1 | |||
| Mpl-L stimulus | |||||||||
| Endogenous TPO (pg/mL) | 324 ± 256 | 926 ± 364 | 1,084 | 1,069 | 328 | ||||
| PEG-rHuMGDF trough (pg/mL) | 1,840 ± 353 | 1,845 | 2,190 | 1,484 | |||||
| Cell counts | |||||||||
| Erythrocytes (×106/μL) | 5.0 ± 0.52 | 5.41 ± 0.47 | 5.2 ± 0.34 | 5.57 | 4.98 | 5.78 | 5.59 | 4.88 | 5.02 |
| Leukocytes (×103/μL) | 12.5 ± 4.6 | 5.2 ± 2.6 | 9.9 ± 5.0 | 5.7 | 9.4 | 2.4 | 5.1 | 7.6 | 15.1 |
| CD4+ T cells (/μL) | >750 | 248 ± 245 | 247 ± 357 | 234 | 85 | 11 | 0 | 500 | 656 |
| Viral load | |||||||||
| Copies (×103/mL) | 0 | 270 ± 260 | 212 ± 172 | 534 | 395 | 260 | 189 | 15 | 53 |
| Determination . | Normal Controls . | HIV-Thrombocytopenic Chimpanzees . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mean Values . | No. 1 . | No. 2 . | No. 3 . | ||||||
| Before Therapy . | 10 d After Therapy . | Before . | After . | Before . | After . | Before . | After . | ||
| Platelets | |||||||||
| Concentration (platelets/μL) | 228 ± 92 | 64 ± 19 | 599 ± 260 | 52 | 547 | 86 | 881 | 60 | 368 |
| MPV (fL) | 10.9 ± 0.7 | 11.2 ± 1.8 | 9.5 ± 0.9 | 12.1 | 10.6 | 9.1 | 8.9 | 12.3 | 9.1 |
| Antiplatelet GPIIIa49-66 ab (×10−3 units) | <20 | 333 ± 278 | 169 ± 164 | 648 | 339 | 123 | 11 | 229 | 157 |
| Megakaryocytes | |||||||||
| No. (×106/kg) | 11 ± 2.1 | 11.7 ± 6.5 | 353 ± 255 | 11 | 182 | 19 | 647 | 5.8 | 231 |
| Volume (×103/fL) | 28.0 ± 4.5 | 38.1 ± 1.9 | 18.7 ± 2.1 | 37 | 20 | 40 | 16 | 37 | 20 |
| Mass (×1010 fL/kg) | 31 ± 5.3 | 45.2 ± 27.2 | 624 ± 373 | 39 | 357 | 75 | 1,050 | 21.4 | 464 |
| Progenitors/103 CD34+ | 3.6 ± 0.6 | 14.1 | 4.4 | 3.2 | 3.2 | 14.1 | |||
| Mpl-L stimulus | |||||||||
| Endogenous TPO (pg/mL) | 324 ± 256 | 926 ± 364 | 1,084 | 1,069 | 328 | ||||
| PEG-rHuMGDF trough (pg/mL) | 1,840 ± 353 | 1,845 | 2,190 | 1,484 | |||||
| Cell counts | |||||||||
| Erythrocytes (×106/μL) | 5.0 ± 0.52 | 5.41 ± 0.47 | 5.2 ± 0.34 | 5.57 | 4.98 | 5.78 | 5.59 | 4.88 | 5.02 |
| Leukocytes (×103/μL) | 12.5 ± 4.6 | 5.2 ± 2.6 | 9.9 ± 5.0 | 5.7 | 9.4 | 2.4 | 5.1 | 7.6 | 15.1 |
| CD4+ T cells (/μL) | >750 | 248 ± 245 | 247 ± 357 | 234 | 85 | 11 | 0 | 500 | 656 |
| Viral load | |||||||||
| Copies (×103/mL) | 0 | 270 ± 260 | 212 ± 172 | 534 | 395 | 260 | 189 | 15 | 53 |