Analyses of Cell Surface Antigens in the Spleen and Lymph Nodes of Treated and Untreated MRL/lpr Mice
| Cell Source . | Cell Surface Antigen (%)* . | H-2 Typing (%) . | |||||
|---|---|---|---|---|---|---|---|
| CD4 . | CD8 . | Thy1.2 . | B220 . | B220/Thy1.2 . | H-2Kk . | H-2Kb . | |
| Untreated MRL/lpr† | |||||||
| Spleen | 17.4 ± 2.1 | 12.4 ± 0.3 | 34.0 ± 9.0 | 16.9 ± 2.3 | 28.2 ± 19.4 | 98.6 ± 0.7 | 0.0 ± 0.0 |
| Lymph nodes | 6.8 ± 0.4 | 4.2 ± 0.2 | 8.2 ± 0.2 | 12.6 ± 2.6 | 78.3 ± 2.5 | 98.3 ± 1.5 | 0.0 ± 0.0 |
| Treated MRL/lpr (protocol 2) | |||||||
| Spleen | 13.4 ± 4.2 | 11.6 ± 4.3 | 26.0 ± 9.7 | 49.0 ± 17.5 | 0.7 ± 0.3 | 0.3 ± 0.2 | 94.2 ± 2.1 |
| Lymph nodes | 25.9 ± 4.8 | 27.4 ± 9.7 | 53.1 ± 15.2 | 34.6 ± 14.3 | 0.8 ± 0.4 | 0.4 ± 0.2 | 97.1 ± 1.2 |
| Cell Source . | Cell Surface Antigen (%)* . | H-2 Typing (%) . | |||||
|---|---|---|---|---|---|---|---|
| CD4 . | CD8 . | Thy1.2 . | B220 . | B220/Thy1.2 . | H-2Kk . | H-2Kb . | |
| Untreated MRL/lpr† | |||||||
| Spleen | 17.4 ± 2.1 | 12.4 ± 0.3 | 34.0 ± 9.0 | 16.9 ± 2.3 | 28.2 ± 19.4 | 98.6 ± 0.7 | 0.0 ± 0.0 |
| Lymph nodes | 6.8 ± 0.4 | 4.2 ± 0.2 | 8.2 ± 0.2 | 12.6 ± 2.6 | 78.3 ± 2.5 | 98.3 ± 1.5 | 0.0 ± 0.0 |
| Treated MRL/lpr (protocol 2) | |||||||
| Spleen | 13.4 ± 4.2 | 11.6 ± 4.3 | 26.0 ± 9.7 | 49.0 ± 17.5 | 0.7 ± 0.3 | 0.3 ± 0.2 | 94.2 ± 2.1 |
| Lymph nodes | 25.9 ± 4.8 | 27.4 ± 9.7 | 53.1 ± 15.2 | 34.6 ± 14.3 | 0.8 ± 0.4 | 0.4 ± 0.2 | 97.1 ± 1.2 |
*Cells from the spleen and lymph nodes (inguinal and axillar regions) were stained with FITC- or PE-conjugated MoAbs, and analyzed using a FACScan.
Numbers represent means ± SDs of 6 mice in each group.