Quantitation of Daudi Cell IFNAR and Dissociation Constants (kd)
| Pretreatment . | DWS Cells . | DWR Cells . | ||
|---|---|---|---|---|
| IFNAR/ Cell . | kd (×10−10) . | IFNAR/ Cell . | kd (×10−10) . | |
| None | 8,620 | 5.5 | 8,940 | 5.2 |
| IFN-α2a-150 | 2,660 | 4.9 | 4,730 | 5.6 |
| Pretreatment . | DWS Cells . | DWR Cells . | ||
|---|---|---|---|---|
| IFNAR/ Cell . | kd (×10−10) . | IFNAR/ Cell . | kd (×10−10) . | |
| None | 8,620 | 5.5 | 8,940 | 5.2 |
| IFN-α2a-150 | 2,660 | 4.9 | 4,730 | 5.6 |
Average of two determinations calculated from Scatchard plots of competitive displacement binding with [125I]-IFN-α2b and cold IFN-α2b (see Materials and Methods).
100 U/mL for 18 hours.