IFN-α2a Effect on Measles Virus Growth Compared in IFN-Sensitive (DWS) and IFN-Resistant (DWR) Daudi Cells
| Virus Assay IFN-α | Virus-Infected Cells | |||||||||||
| DWS | DWR | |||||||||||
| Supernate* | Cell† | ICA‡ | Supernate* | Cell† | ICA‡ | |||||||
| − | + | − | + | − | + | − | + | − | + | − | + | |
| Infectivity | 4.7 | 2.5 | 3.7 | 1.5 | 29 | 6 | 4.2 | 4.5 | 2.7 | 3.0 | 21 | 18 |
| % Inhibition | 99 | 99 | 70 | 0 | 0 | 14 | ||||||
| Virus Assay IFN-α | Virus-Infected Cells | |||||||||||
| DWS | DWR | |||||||||||
| Supernate* | Cell† | ICA‡ | Supernate* | Cell† | ICA‡ | |||||||
| − | + | − | + | − | + | − | + | − | + | − | + | |
| Infectivity | 4.7 | 2.5 | 3.7 | 1.5 | 29 | 6 | 4.2 | 4.5 | 2.7 | 3.0 | 21 | 18 |
| % Inhibition | 99 | 99 | 70 | 0 | 0 | 14 | ||||||
Cells incubated with or without IFNα2a (100 U/mL) for 24 hours before infection with Edmonston strain measles virus (1 TCID50/cell).
Log10 titers (TCID50 per milliliter) of virus released at 48 hours, detected by microtitration of culture fluids on VERO cells for 6 days.
Log10 titers (TCID50 per milliliter) of virus that was cell-associated at 48 hours, detected by microtitration of whole cell lysates on VERO cells for 6 days.
Infectious center assay of Daudi cells infected with measles virus for 48 hours. Data represent the ratio (×100) of virus plaques enumerated per number of the Daudi cells inoculated onto indicator cell (VERO) monolayers.