ATA Reduction of IFN-α Antiviral Activity in IFN-Sensitive (DWS) Daudi Cells
| ATA3-150 @ Time . | Relative Reduction of IFN-α Activity (%) . | |
|---|---|---|
| Virus Assay . | ||
| Supernatant3-151 . | ICA3-152 . | |
| 4 h | 100 | 44 |
| 18 h | 59 | 0 |
| ATA3-150 @ Time . | Relative Reduction of IFN-α Activity (%) . | |
|---|---|---|
| Virus Assay . | ||
| Supernatant3-151 . | ICA3-152 . | |
| 4 h | 100 | 44 |
| 18 h | 59 | 0 |
Cells were incubated in growth medium with IFN-α (100 U/mL) for 24 hours, washed, and infected with the Edmonston strain measles virus (1 TCID50/cell).
ATA (125 μmol/L) was added to the medium with IFN-α at the times indicated.
Log10 virus titers (TCID50) of supernatant released virus at 48 hours after infection of the DWS cells. Yield of infectious virus was determined by microtitration on VERO cells for 6 days. In control DWS cells treated with ATA compared with or without IFN-α at 0 hours of infection, the ATA caused a reduction of 75% to 90% of the IFN-α antiviral activity. For experimental normalization, the 0 hours results define a 100% basis for all data shown in this table. The true effect of ATA added at 0 hours was a 75% reduction in antiviral activity (data not shown).
Data represent the ratio (×100) of viral plaques enumerated on monolayers of indicator cells (VERO) per number of infected Daudi cells inoculated.