Comparison of Current IgG Fab/Phage Library-Derived Anti-Rh(D) MoAbs With Those Previously Produced by Conventional Tissue Culture Methods
| Attribute . | Previously Published* . | Current Study . | |
|---|---|---|---|
| Heavy Chains | (by clone)-151 | (by VDJ) | |
| VH3 family derived | 12/21 (57%) | 28/28 (100%) | 12/12 (100%) |
| VH3-33 superspecies-152/VH3 | 10/12 (83%) | 26/28 (93%) | 11/12 (92%) |
| VH3-33/VH3 | 9/12 (75%) | 19/28 (68%) | 9/12 (75%) |
| VH3-21/VH3 | 1/12 (8%) | 2/28 (7%) | 1/12 (8%) |
| VH4-34 derived | 2/21 (10%) | 0/28 (0%) | 0/12 (0%) |
| JH6 usage | 15/21 (71%) | 9/28 (32%) | 5/12 (42%) |
| CDR3 length | 16-20 (28-153) | 15-19 | |
| κ Light Chains | |||
| Vκ1 family derived/total κ | 8/12 (67%) | 17/18 (94%) | |
| Jκ1 usage/total κ | 4/12 (33%) | 6/18 (33%) | |
| Jκ2 usage/total κ | 4/12 (33%) | 6/18 (33%) | |
| λ Light Chains | |||
| Vλ1 family derived/total λ | 2/8 (25%) | 12/23 (52%) | |
| Vλ3 family derived/total λ | 5/8 (63%) | 5/23 (22%) | |
| DPL16 derived/Vλ3 family | 3/5 (60%) | 4/5 (80%) | |
| Jλ2Vasicek usage/total λ | 6/8 (75%) | 23/23 (100%) | |
| Attribute . | Previously Published* . | Current Study . | |
|---|---|---|---|
| Heavy Chains | (by clone)-151 | (by VDJ) | |
| VH3 family derived | 12/21 (57%) | 28/28 (100%) | 12/12 (100%) |
| VH3-33 superspecies-152/VH3 | 10/12 (83%) | 26/28 (93%) | 11/12 (92%) |
| VH3-33/VH3 | 9/12 (75%) | 19/28 (68%) | 9/12 (75%) |
| VH3-21/VH3 | 1/12 (8%) | 2/28 (7%) | 1/12 (8%) |
| VH4-34 derived | 2/21 (10%) | 0/28 (0%) | 0/12 (0%) |
| JH6 usage | 15/21 (71%) | 9/28 (32%) | 5/12 (42%) |
| CDR3 length | 16-20 (28-153) | 15-19 | |
| κ Light Chains | |||
| Vκ1 family derived/total κ | 8/12 (67%) | 17/18 (94%) | |
| Jκ1 usage/total κ | 4/12 (33%) | 6/18 (33%) | |
| Jκ2 usage/total κ | 4/12 (33%) | 6/18 (33%) | |
| λ Light Chains | |||
| Vλ1 family derived/total λ | 2/8 (25%) | 12/23 (52%) | |
| Vλ3 family derived/total λ | 5/8 (63%) | 5/23 (22%) | |
| DPL16 derived/Vλ3 family | 3/5 (60%) | 4/5 (80%) | |
| Jλ2Vasicek usage/total λ | 6/8 (75%) | 23/23 (100%) | |
*Compiled from a total of 21 sequences of IgG anti-Rh(D) antibodies isolated from multiple subjects originally published by Bye et al,25 Hughes-Jones et al,23 Chouchane et al,24 and Boucher et al10 and available from Genbank. One light chain (Oak-3)25 was not available in Genbank and was not included in the assessment.
For heavy chains, the left column tabulates each clone separately and the right column tabulates clones on the basis of shared V-D-J joining regions.
VH3-33 superspecies defined as the group of VH3 family germline genes comprising VH3-33, VH3-30, and VH30.3.
CDR3 length outlier.