Effects of GVHD on the Number of Hematopoietic Progenitor Cells and Fas Expression
| . | n . | Days After Transfer . | No. of Lin−c-kit+ Cells . | c-kit+Fas+ in Lin− . | ||
|---|---|---|---|---|---|---|
| ×104 . | P Value . | % . | P Value . | |||
| Exp 1 | ||||||
| BDF1 | 4 | — | 52.8 ± 4.5 | — | 3.1 ± 0.4 | — |
| B6/BDF1 | 4 | 14 | 12.5 ± 1.2 | .0009 | 20.8 ± 4.0 | .0035 |
| Exp 2 | ||||||
| BDF1 | 4 | — | 53.7 ± 2.0 | — | 2.6 ± 0.3 | — |
| B6/BDF1 | 4 | 14 | 12.6 ± 2.3 | .0048 | 24.2 ± 2.5 | .0005 |
| Exp 3 | ||||||
| BDF1 | 4 | — | 56.1 ± 3.3 | — | 2.8 ± 0.2 | — |
| B6/BDF1 | 4 | 14 | 7.5 ± 1.5 | .0001 | 20.8 ± 2.7 | .0015 |
| D2/BDF1 | 4 | 14 | 57.9 ± 5.7 | .4248 | 1.9 ± 0.6 | .1159 |
| Exp 4 | ||||||
| BDF1 | 5 | — | 55.2 ± 0.8 | — | 2.5 ± 0.2 | — |
| B6/BDF1 | 5 | 10 | 33.8 ± 2.9 | .0029 | 7.9 ± 1.7 | .0026 |
| . | n . | Days After Transfer . | No. of Lin−c-kit+ Cells . | c-kit+Fas+ in Lin− . | ||
|---|---|---|---|---|---|---|
| ×104 . | P Value . | % . | P Value . | |||
| Exp 1 | ||||||
| BDF1 | 4 | — | 52.8 ± 4.5 | — | 3.1 ± 0.4 | — |
| B6/BDF1 | 4 | 14 | 12.5 ± 1.2 | .0009 | 20.8 ± 4.0 | .0035 |
| Exp 2 | ||||||
| BDF1 | 4 | — | 53.7 ± 2.0 | — | 2.6 ± 0.3 | — |
| B6/BDF1 | 4 | 14 | 12.6 ± 2.3 | .0048 | 24.2 ± 2.5 | .0005 |
| Exp 3 | ||||||
| BDF1 | 4 | — | 56.1 ± 3.3 | — | 2.8 ± 0.2 | — |
| B6/BDF1 | 4 | 14 | 7.5 ± 1.5 | .0001 | 20.8 ± 2.7 | .0015 |
| D2/BDF1 | 4 | 14 | 57.9 ± 5.7 | .4248 | 1.9 ± 0.6 | .1159 |
| Exp 4 | ||||||
| BDF1 | 5 | — | 55.2 ± 0.8 | — | 2.5 ± 0.2 | — |
| B6/BDF1 | 5 | 10 | 33.8 ± 2.9 | .0029 | 7.9 ± 1.7 | .0026 |
The BDF1 mice were injected with 5 × 107 of B6 spleen cells (B6/BDF1) or 1 × 108 of D2 cells (D2/BDF1). The number of Lin−c-kit+ cells and c-kit+Fas+ fraction of Lin− cells were examined on day 10 or 14 after transfer of spleen cells. The results from four to five mice were expressed as the mean ± SEM. P value were calculated when compared with uninjected control BDF1 mice.