Quantitation of F. IX Message by Laser Densitometric Scanning
| Lane . | Area (AU × mm) . | Normalized Message (1):(2) . | |
|---|---|---|---|
| F. IX Transcript (1) . | GAPDH Transcript (2) . | ||
| 1 | 2.25 | 0.58 | 3.87 |
| 2 | 1.22 | 0.59 | 2.06 |
| 3 | 0.23 | 0.93 | 0.24 |
| 4 | 0.23 | 0.73 | 0.31 |
| 5 | 2.48 | 0.69 | 3.59 |
| 6 | 0.83 | 1.62 | 0.51 |
| 7 | 2.95 | 1.54 | 1.91 |
| Lane . | Area (AU × mm) . | Normalized Message (1):(2) . | |
|---|---|---|---|
| F. IX Transcript (1) . | GAPDH Transcript (2) . | ||
| 1 | 2.25 | 0.58 | 3.87 |
| 2 | 1.22 | 0.59 | 2.06 |
| 3 | 0.23 | 0.93 | 0.24 |
| 4 | 0.23 | 0.73 | 0.31 |
| 5 | 2.48 | 0.69 | 3.59 |
| 6 | 0.83 | 1.62 | 0.51 |
| 7 | 2.95 | 1.54 | 1.91 |
All of the lanes from 1 to 7 of Fig 5A were scanned by Laser Densitometer (Ultroscan XL; LKB, Bromma, Sweden). The F. IX major transcript band was considered in each case. The area for the background level was 0.20. AU, absorbance unit, height is expressed in AU; position is expressed in mm.