Granulocyte-Macrophage Colony-Forming Unit Analysis
| Experimental Conditions . | Ad. CMV-CD (50 MOI) . | 5-FC (μmol/L) . | Colonies . | |
|---|---|---|---|---|
| Exp. 1 . | Exp. 2 . | |||
| CD34 | − | 10 | 734 | 756 |
| CD34 | − | 50 | 744 | 724 |
| CD34 + 0.1% MCF-7 | − | 10 | 750 | 796 |
| CD34 + 0.1% MCF-7 | − | 50 | 761 | 722 |
| CD34 | + | 10 | 766 | 772 |
| CD34 | + | 50 | 880 | 846 |
| CD34 + 0.1% MCF-7 | + | 10 | 824 | 860 |
| CD34 + 0.1% MCF-7 | + | 50 | 684 | 630 |
| Experimental Conditions . | Ad. CMV-CD (50 MOI) . | 5-FC (μmol/L) . | Colonies . | |
|---|---|---|---|---|
| Exp. 1 . | Exp. 2 . | |||
| CD34 | − | 10 | 734 | 756 |
| CD34 | − | 50 | 744 | 724 |
| CD34 + 0.1% MCF-7 | − | 10 | 750 | 796 |
| CD34 + 0.1% MCF-7 | − | 50 | 761 | 722 |
| CD34 | + | 10 | 766 | 772 |
| CD34 | + | 50 | 880 | 846 |
| CD34 + 0.1% MCF-7 | + | 10 | 824 | 860 |
| CD34 + 0.1% MCF-7 | + | 50 | 684 | 630 |
Cells were exposed for 48 hours at the above-described conditions. Three thousand nucleated cells were plated in each 35-mm dish with 1.3 mL methylcellulose and colonies were counted after 12 to 14 days. A colony is defined as 50 cells or greater. (See Hanania et al25 for details of the assay.)