Colony-Forming Assay Using Fetal Liver Cells at E13.0
. | n . | Total . | E . | Mix . | GM . |
---|---|---|---|---|---|
A. | |||||
+/+ | 1 | 4,984 | 336 | 280 | 4,368 |
+/mu | 3 | 2,514 ± 787∥ | 276 ± 43‡ | 69 ± 38¶ | 2,184 ± 752# |
mu/mu | 3 | 148 ± 47∥ | 66 ± 51‡ | 16 ± 15¶ | 66 ± 13# |
B. | |||||
+/+ | 1 | 8,400 | 1,960 | 168 | 6,384 |
+/mu | 3 | 5,640 ± 650* | 1,228 ± 247§ | 86 ± 76 | 4,635 ± 387† |
mu/mu | 3 | 543 ± 250* | 214 ± 175§ | 16 ± 17 | 313 ± 64† |
. | n . | Total . | E . | Mix . | GM . |
---|---|---|---|---|---|
A. | |||||
+/+ | 1 | 4,984 | 336 | 280 | 4,368 |
+/mu | 3 | 2,514 ± 787∥ | 276 ± 43‡ | 69 ± 38¶ | 2,184 ± 752# |
mu/mu | 3 | 148 ± 47∥ | 66 ± 51‡ | 16 ± 15¶ | 66 ± 13# |
B. | |||||
+/+ | 1 | 8,400 | 1,960 | 168 | 6,384 |
+/mu | 3 | 5,640 ± 650* | 1,228 ± 247§ | 86 ± 76 | 4,635 ± 387† |
mu/mu | 3 | 543 ± 250* | 214 ± 175§ | 16 ± 17 | 313 ± 64† |
(A) Numbers of colonies per total liver at 7th day of culture. (B) Numbers of colonies per total liver at 14th day of culture.
Abbreviations: E, erythroid colony; Mix, mixed colony; GM, granulocyte-macrophage colony; n, number of embryos analyzed; +/+, Mll+/+; +/mu, Mll+/mu; mu/mu, Mllmu/mu.
Statistically significant difference at *†<.001; ‡§<.01; ∥¶#<.05. Statistical analysis was performed by Student'st-test and Welch's t-test.