Table 5.

Colony-Forming Assay Using Fetal Liver Cells at E13.0

n Total E Mix GM
A.  
 +/+  4,984    336  280  4,368 
 +/mu  3  2,514 ± 787 276 ± 43 69 ± 38¶  2,184 ± 752# 
 mu/mu  3  148 ± 47 66 ± 51 16 ± 15¶  66 ± 13#  
B.  
 +/+  8,400  1,960  168  6,384 
 +/mu  3  5,640 ± 650* 1,228 ± 247§ 86 ± 76  4,635 ± 387 
 mu/mu  543 ± 250* 214 ± 175§ 16 ± 17 313 ± 64 
n Total E Mix GM
A.  
 +/+  4,984    336  280  4,368 
 +/mu  3  2,514 ± 787 276 ± 43 69 ± 38¶  2,184 ± 752# 
 mu/mu  3  148 ± 47 66 ± 51 16 ± 15¶  66 ± 13#  
B.  
 +/+  8,400  1,960  168  6,384 
 +/mu  3  5,640 ± 650* 1,228 ± 247§ 86 ± 76  4,635 ± 387 
 mu/mu  543 ± 250* 214 ± 175§ 16 ± 17 313 ± 64 

(A) Numbers of colonies per total liver at 7th day of culture. (B) Numbers of colonies per total liver at 14th day of culture.

Abbreviations: E, erythroid colony; Mix, mixed colony; GM, granulocyte-macrophage colony; n, number of embryos analyzed; +/+, Mll+/+; +/mu, Mll+/mu; mu/mu, Mllmu/mu.

Statistically significant difference at *†<.001; ‡§<.01; ∥¶#<.05. Statistical analysis was performed by Student'st-test and Welch's t-test.

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