Chromosomal Alteration Included in the Multiplex RT-PCR Analysis
| Chromosomal Alteration . | Genes Involved . | Fusion Gene* . | PCR Mix No.† . | Size of PCR Fragment‡ . | Positive Cell Line . | Presence . | Reference . |
|---|---|---|---|---|---|---|---|
| t(X;11)(q13q23) | MLL (11q23) | MLLex6/AFX | R1C | 488 | T | ||
| AFX (Xq13) | MLLex7/AFX | R1C | 235 (480) | T | |||
| MLLex8/AFX | R1C | 449 (694) | Karpas-45§ | ALL | 7 | ||
| MLLex9/AFX | R1C | 596 (841) | T | ||||
| TAL1D | SIL (1p34) | SIL/TAL1 | R3D | 183 | RPMI8402 | T-ALL | 32, 37 |
| TAL1 (1p34) | |||||||
| t(1;11)(q21;q23) | MLL (11q23) | MLLex6/AF1q | R5E | 300 | AMMOL | 13 | |
| AF1q (1q21) | MLLex7/AF1q | R5E | 187 | T | |||
| MLLex8/AF1q | R5E | 301 (546) | T | ||||
| MLLex9/AF1q | R5E | 448 (693) | T | ||||
| t(1;11)(p32;q23) | MLL (11q23) | MLLex6/AF-qp | R2A | 300 | ALL | 10 | |
| AF1p (1p32) | MLLex7/AF-1p | R2A | 187 (432) | T | |||
| MLLex8/AF-1p | R2A | 301 (546) | T | ||||
| MLLex9/AF-1p | R2A | 448 (693) | T | ||||
| t(1;19)(q23;p13) | E2A(19p13) | E2A/PBX1 (1) | R3A | 376 | 697 | ALL | 28, 38, 39 |
| PBX1 (1q23) | E2A/PBX1 (1a) | R3A | 403 | ALL | 39 | ||
| t(2;5)(p23;q35) | NPM(5q35) | NPM/ALK | R8D | 302 | Karpas-299 | ALCL, T-/B-cell lymphomas | 40, 41 |
| ALK (2p23) | |||||||
| t(3;5)(q25.1;q34) | NPM (5q35) | NPM/MLF1 | R8F | 289 | MDS, AML | 42 | |
| MLF1 (3q25.1) | |||||||
| t(3;21)(q26;q22) | AML1 (21q22) | AML1ex5/MDS1/(EVI1) | R4B | 446 | SKH1§ | CML-BC, AML, MDS | 43-45 |
| MDS1 (3q26) | |||||||
| (EVI1) (3q26) | AML1ex6/MDS1/(EVI1) | R4B | 638 | CML-BC, AML, MDS | 44, 45 | ||
| t(4;11)(q21;q23) | MLL (11q23) | MLLex6/AF4 (a:1414) | R5A | 317 | ALL | 14, 16, 46 | |
| AF4(4q21) | MLLex7/AF4 (a:1414) | R5A | 204 (449) | RS4; 11 | ALL | 14, 16, 46 | |
| MLLex8/AF4 (a:1414) | R5A | 318 (563) | ALL | 16, 17, 46 | |||
| MLLex8/AF4 (a:1414) | R5A | 465 (710) | T | ||||
| MLLex6/AF4(b:1459) | R5A | 272 | MV-4-11 | ALL | 17, 46 | ||
| MLLex7/AF4 (b:1459) | R5A | 159 (404) | ALL | 14, 17, 46 | |||
| MLLex8/AF4 (b:1459) | R5A | 273 (518) | ALL | 14, 17 | |||
| MLLex9/AF4 (b:1459) | R5A | 420 (665) | T | ||||
| MLLex6/AF4(c:1546) | R5A | 185 | T | ||||
| MLLex7/AF4 (c:1546) | R5A | 72 (317) | ALL | 17 | |||
| MLLex8/AF4 (c:1546) | R5A | 186 (431) | ALL | 14, 17, 46 | |||
| MLLex9/AF4 (c:1546) | R5A | 333 (578) | T | ||||
| t(5;12)(q33;p13) | TEL(12p13) | TEL/PDGFR | R6D | 321 | CMML, MDS | 47 | |
| PDGFR (5q33) | |||||||
| t(5;17)(q35;q22) | NPM(5q35) | NPM(S)/RAR | R8E | 105 | APL | 48 | |
| RAR (17q21) | NPM(L)/RAR | R8E | 234 | APL | 48 | ||
| t(6;9)(p23;q34) | DEK(6p23) | DEK/CAN | R7A | 320 | AML | 49 | |
| CAN (9q34) | |||||||
| t(6;11)(q27;q23) | MLL (11q23) | MLLex6/AF6 | R1D | 308 | ML-2 | AML, ALL¶ | 8, 20 |
| AF6 (6q27) | MLLex7/AF6 | R1D | 195 (440) | AML | 20 | ||
| MLLex8/AF6 | R1D | 309 (594) | T | ||||
| MLLex9/AF6 | R1D | 456 (741) | T | ||||
| t(7;10)(q35;q24) | Activation of | HOX11□ | R4D | 212 | RPMI8402 | T-ALL | 50, 51 |
| t(8;21)(q22;q22) | AML1 (21q22) | AMLex5/ETO | R4A | 353 | Kasumi-1§ | AML | 5, 45, 52 |
| ETO (8q22) | |||||||
| ?t(9;9) | SET(9q34) | SET/CAN | R7B | 393 | AUL | 53 | |
| CAN (9q34) | |||||||
| t(9;11)(p22;q23) | MLL (11q23) | MLLex6/AF9 (A) | R5C | 321 | AML | 14 | |
| AF9 (9p22) | MLLex7/AF9 (A) | R5C | 208 (453) | Mono-Mac-6 | AML | 14 | |
| MLLex8/AF9 (A) | R5C | 322 (567) | Mono-Mac-6 | AML | 14 | ||
| MLLex9/AF9 (A) | R5C | 469 (714) | T | ||||
| MLLex6/AF9(B) | R5D | 367 | AML | 19, 20 | |||
| MLLex7/AF9 (B) | R5D | 254 (499) | T | ||||
| MLLex8/AF9(B) | R5D | 368 (613) | T | ||||
| MLLex9/AF9 (B) | R5D | 515 (760) | T | ||||
| t(9;12)(q34;p13) | TEL(12p13) | TEL/ABL | R6C | 366 | ALL | 54 | |
| ABL (9q34) | |||||||
| t(9;22)(q34;q11) | BCR (22q11) | BCR/ABL e1a2 | R6A | 320 | ALL | 23, 55 | |
| ABL (9q34) | BCR/ABL b2a2 | R6B | 472 | CML | 23, 55 | ||
| BCR/ABL b3a2 | R6B | 397 | CML | 23, 55 | |||
| t(10;11)(p12;q23) | MLL (11q23) | MLLex5/AF10 (A;2222) | R2C | 202 | AML | 56, 57 | |
| AF10 (10p12) | MLLex6/AF10(B:979) | R2D | 270 | AML | 56, 57 | ||
| MLLex7/AF10 (B:979) | R2D | 157 (402) | AML | 57 | |||
| MLLex8/AF10 (B:979) | R2D | 271 (516) | T | ||||
| MLLex9/AF10(B:979) | R2D | 418 (663) | T | ||||
| MLLex6/AF10 (C:2110) | R2C | 388 | AML | 56, 57 | |||
| MLLex7/AF10 (C:2110) | R2C | 275 (520) | T | ||||
| MLLex8/AF10 (C:2110) | R2C | 389 (634) | T | ||||
| MLLex9/AF10(C:2110) | R2C | 536 (781) | T | ||||
| MLLex6/AF10 (D:883) | R2D | 366 | AML | 57 | |||
| MLLex7/AF10 (D:883) | R2D | 253 (498) | AML | 57 | |||
| MLLex8/AF10 (D:883) | R2D | 367 (612) | T | ||||
| MLLex9/AF10(D:883) | R2D | 514 (759) | T | ||||
| MLLex6/AF10 (E:589) | R2E | 267 | AML | 57 | |||
| MLLex7/AF10 (E:589) | R2E | 154 (399) | T | ||||
| MLLex8/AF10 (E:589) | R2E | 268 (513) | T | ||||
| MLLex9/AF10(E:589) | R2E | 415 (660) | T | ||||
| MLLex5/AF10 (F:1931) | R2C | 493 | AML¶ | ||||
| t(10;14)(q24;q11) | HOX11 (10q24) | Karpas-299 | AML, ALL, CML¶ | ||||
| dupMLL (11q23) | MLL (11q23) | MLLex5/MLLex2 | R2F | 184 | ALL¶ | ||
| MLL (11q23) | MLLex6/MLLex2 | R2F | 258 | AML, ALL | 15, 20, 21 | ||
| MLLex7/MLLex2 | R2F | 145 (390) | AML | 21 | |||
| MLLex8/MLLex2 | R2F | 259 (504) | AML | 21 | |||
| MLLex9/MLLex2 | R2F | 406 (651) | AML | 21 | |||
| t(11;17)(q23;q21) | PLZF (11q23) | PLZF/RAR (A:1365) | R8A | 315 | APL | 58, 59 | |
| RARA (17q21) | PLZF/RAR(B:1452) | R8A | 402 | APL | 59 | ||
| t(11;17)(q23;q21) | MLL (11q23) | MLLex5/AF17 | R2B | 281 | AML | 11 | |
| AF17 (17q21) | |||||||
| t(11;19)(q23;p13.1) | MLL (11q23) | MLLex6/ELL | R1E | 330 | T | ||
| ELL (19p13.1) | MLLex7/ELL | R1E | 217 (462) | AML | 9, 60, 61 | ||
| MLLex8/ELL | R1E | 301 (576) | T | ||||
| MLLex9/ELL | R1E | 448 (723) | T | ||||
| MLLex6/ELL-ins120 | R1E | 450 | T | ||||
| MLLex7/ELL-ins120 | R1E | 337 (582) | AML | 60 | |||
| MLLex8/ELL-ins120 | R1E | 451 (696) | AML | 60 | |||
| MLLex9/ELL-ins120 | R1E | 598 (845) | T | ||||
| t(11;19)(q23;p13.3) | MLL(11q23) | MLLex6/ENL (A:177) | R5B | 186 | ALL | 14, 17 | |
| ENL (19p13.3) | MLLex7/ENL (A:177) | R5B | 73 (318) | KOCL-44§ | ALL | 14, 17 | |
| MLLex8/ENL(A:177) | R5B | 187 (432) | KOCL-44§ | ALL | 14, 17 | ||
| MLLex9/ENL (A:177) | R5B | 334 (579) | T | ||||
| t(12;21)(p13;q22) | TEL(12p13) | TEL/AML1 | R3C | 293 | ALL | 62 | |
| AML1 (21q22) | TEL/AML1 | R3C | 332 | ALL | 62-64 | ||
| t(15;17)(q21;q22) | PML (15q21) | PMLex3/RARα ex2 | R8C | 393 | NB4 | APL | 65-67 |
| RAR (17q21) | S-form (=BCR3) | ||||||
| PMLex3/RARα ex2 | R8C | 338 | NB4 | APL | 66 | ||
| S-form splice variant | |||||||
| PMLex6/RARα ex2 | R8B | 427 | NB4 | APL | 66, 67 | ||
| L-form (=BCR1) | |||||||
| PMLa ex3 Δex5 + 6/ | R8C | 464 | NB4 | APL | 67 | ||
| RARα ex2, | |||||||
| L-form splice variant | |||||||
| PML ex6-(+/−)ins- | R8B | +/−427 | APL | 66 | |||
| RAR ex2 | |||||||
| V-form (=BCR2) | |||||||
| inv(16)(p13q22) | CBF (16q22) | CBF/MYH11 (A) | R1A | 270 | ME-1§ | AML | 68 |
| MYH11 (16p13) | CBF/MYH11 (B) | R1B | 483 | AML | 68 | ||
| CBF/MYH11 (C) | R1B | 663 | AML | 68 | |||
| CBF/MYH11 (D) | R1A | 337 | AML | 68 | |||
| CBF/MYH11 (E) | R1A | 544 | AML | 68 | |||
| CBF/MYH11 (F) | R1B | 174 | AML | 68 | |||
| CBF/MYH11 (G) | R1B | 241 | AML | 68 | |||
| CBF/MYH11 (H) | R1B | 348 | AML | 68 | |||
| t(16;21)(p11;q22) | TLS (16p11) | TLS/ERG (a) | R4C | 318 | UTP-L12§ | AML | 69 |
| ERG (21q22) | TLS/ERG (b) | R4C | 274 | UTP-L12§ | AML | 69 | |
| TLS/ERG (c) | R4C | 239 | UTP-L12§ | AML, ALL | 69 | ||
| TLS/ERG(d) | R4C | 344 | ALL¶ | ||||
| TLS/ERG (e) | R4C | 413 | ALL¶ | ||||
| t(17;19)(q22;p13) | E2A(19p13) | E2Aex13/HLFex4 (I) | R3B | 390 | HAL-01 | ALL | 70-72 |
| HLF (17q22) | E2Aex13insHLFex4 (I) | R3B | 417 | ALL | 72 | ||
| E2Aex12/HLFex4 (II) | R3B | 207 | ALL | 72 |
| Chromosomal Alteration . | Genes Involved . | Fusion Gene* . | PCR Mix No.† . | Size of PCR Fragment‡ . | Positive Cell Line . | Presence . | Reference . |
|---|---|---|---|---|---|---|---|
| t(X;11)(q13q23) | MLL (11q23) | MLLex6/AFX | R1C | 488 | T | ||
| AFX (Xq13) | MLLex7/AFX | R1C | 235 (480) | T | |||
| MLLex8/AFX | R1C | 449 (694) | Karpas-45§ | ALL | 7 | ||
| MLLex9/AFX | R1C | 596 (841) | T | ||||
| TAL1D | SIL (1p34) | SIL/TAL1 | R3D | 183 | RPMI8402 | T-ALL | 32, 37 |
| TAL1 (1p34) | |||||||
| t(1;11)(q21;q23) | MLL (11q23) | MLLex6/AF1q | R5E | 300 | AMMOL | 13 | |
| AF1q (1q21) | MLLex7/AF1q | R5E | 187 | T | |||
| MLLex8/AF1q | R5E | 301 (546) | T | ||||
| MLLex9/AF1q | R5E | 448 (693) | T | ||||
| t(1;11)(p32;q23) | MLL (11q23) | MLLex6/AF-qp | R2A | 300 | ALL | 10 | |
| AF1p (1p32) | MLLex7/AF-1p | R2A | 187 (432) | T | |||
| MLLex8/AF-1p | R2A | 301 (546) | T | ||||
| MLLex9/AF-1p | R2A | 448 (693) | T | ||||
| t(1;19)(q23;p13) | E2A(19p13) | E2A/PBX1 (1) | R3A | 376 | 697 | ALL | 28, 38, 39 |
| PBX1 (1q23) | E2A/PBX1 (1a) | R3A | 403 | ALL | 39 | ||
| t(2;5)(p23;q35) | NPM(5q35) | NPM/ALK | R8D | 302 | Karpas-299 | ALCL, T-/B-cell lymphomas | 40, 41 |
| ALK (2p23) | |||||||
| t(3;5)(q25.1;q34) | NPM (5q35) | NPM/MLF1 | R8F | 289 | MDS, AML | 42 | |
| MLF1 (3q25.1) | |||||||
| t(3;21)(q26;q22) | AML1 (21q22) | AML1ex5/MDS1/(EVI1) | R4B | 446 | SKH1§ | CML-BC, AML, MDS | 43-45 |
| MDS1 (3q26) | |||||||
| (EVI1) (3q26) | AML1ex6/MDS1/(EVI1) | R4B | 638 | CML-BC, AML, MDS | 44, 45 | ||
| t(4;11)(q21;q23) | MLL (11q23) | MLLex6/AF4 (a:1414) | R5A | 317 | ALL | 14, 16, 46 | |
| AF4(4q21) | MLLex7/AF4 (a:1414) | R5A | 204 (449) | RS4; 11 | ALL | 14, 16, 46 | |
| MLLex8/AF4 (a:1414) | R5A | 318 (563) | ALL | 16, 17, 46 | |||
| MLLex8/AF4 (a:1414) | R5A | 465 (710) | T | ||||
| MLLex6/AF4(b:1459) | R5A | 272 | MV-4-11 | ALL | 17, 46 | ||
| MLLex7/AF4 (b:1459) | R5A | 159 (404) | ALL | 14, 17, 46 | |||
| MLLex8/AF4 (b:1459) | R5A | 273 (518) | ALL | 14, 17 | |||
| MLLex9/AF4 (b:1459) | R5A | 420 (665) | T | ||||
| MLLex6/AF4(c:1546) | R5A | 185 | T | ||||
| MLLex7/AF4 (c:1546) | R5A | 72 (317) | ALL | 17 | |||
| MLLex8/AF4 (c:1546) | R5A | 186 (431) | ALL | 14, 17, 46 | |||
| MLLex9/AF4 (c:1546) | R5A | 333 (578) | T | ||||
| t(5;12)(q33;p13) | TEL(12p13) | TEL/PDGFR | R6D | 321 | CMML, MDS | 47 | |
| PDGFR (5q33) | |||||||
| t(5;17)(q35;q22) | NPM(5q35) | NPM(S)/RAR | R8E | 105 | APL | 48 | |
| RAR (17q21) | NPM(L)/RAR | R8E | 234 | APL | 48 | ||
| t(6;9)(p23;q34) | DEK(6p23) | DEK/CAN | R7A | 320 | AML | 49 | |
| CAN (9q34) | |||||||
| t(6;11)(q27;q23) | MLL (11q23) | MLLex6/AF6 | R1D | 308 | ML-2 | AML, ALL¶ | 8, 20 |
| AF6 (6q27) | MLLex7/AF6 | R1D | 195 (440) | AML | 20 | ||
| MLLex8/AF6 | R1D | 309 (594) | T | ||||
| MLLex9/AF6 | R1D | 456 (741) | T | ||||
| t(7;10)(q35;q24) | Activation of | HOX11□ | R4D | 212 | RPMI8402 | T-ALL | 50, 51 |
| t(8;21)(q22;q22) | AML1 (21q22) | AMLex5/ETO | R4A | 353 | Kasumi-1§ | AML | 5, 45, 52 |
| ETO (8q22) | |||||||
| ?t(9;9) | SET(9q34) | SET/CAN | R7B | 393 | AUL | 53 | |
| CAN (9q34) | |||||||
| t(9;11)(p22;q23) | MLL (11q23) | MLLex6/AF9 (A) | R5C | 321 | AML | 14 | |
| AF9 (9p22) | MLLex7/AF9 (A) | R5C | 208 (453) | Mono-Mac-6 | AML | 14 | |
| MLLex8/AF9 (A) | R5C | 322 (567) | Mono-Mac-6 | AML | 14 | ||
| MLLex9/AF9 (A) | R5C | 469 (714) | T | ||||
| MLLex6/AF9(B) | R5D | 367 | AML | 19, 20 | |||
| MLLex7/AF9 (B) | R5D | 254 (499) | T | ||||
| MLLex8/AF9(B) | R5D | 368 (613) | T | ||||
| MLLex9/AF9 (B) | R5D | 515 (760) | T | ||||
| t(9;12)(q34;p13) | TEL(12p13) | TEL/ABL | R6C | 366 | ALL | 54 | |
| ABL (9q34) | |||||||
| t(9;22)(q34;q11) | BCR (22q11) | BCR/ABL e1a2 | R6A | 320 | ALL | 23, 55 | |
| ABL (9q34) | BCR/ABL b2a2 | R6B | 472 | CML | 23, 55 | ||
| BCR/ABL b3a2 | R6B | 397 | CML | 23, 55 | |||
| t(10;11)(p12;q23) | MLL (11q23) | MLLex5/AF10 (A;2222) | R2C | 202 | AML | 56, 57 | |
| AF10 (10p12) | MLLex6/AF10(B:979) | R2D | 270 | AML | 56, 57 | ||
| MLLex7/AF10 (B:979) | R2D | 157 (402) | AML | 57 | |||
| MLLex8/AF10 (B:979) | R2D | 271 (516) | T | ||||
| MLLex9/AF10(B:979) | R2D | 418 (663) | T | ||||
| MLLex6/AF10 (C:2110) | R2C | 388 | AML | 56, 57 | |||
| MLLex7/AF10 (C:2110) | R2C | 275 (520) | T | ||||
| MLLex8/AF10 (C:2110) | R2C | 389 (634) | T | ||||
| MLLex9/AF10(C:2110) | R2C | 536 (781) | T | ||||
| MLLex6/AF10 (D:883) | R2D | 366 | AML | 57 | |||
| MLLex7/AF10 (D:883) | R2D | 253 (498) | AML | 57 | |||
| MLLex8/AF10 (D:883) | R2D | 367 (612) | T | ||||
| MLLex9/AF10(D:883) | R2D | 514 (759) | T | ||||
| MLLex6/AF10 (E:589) | R2E | 267 | AML | 57 | |||
| MLLex7/AF10 (E:589) | R2E | 154 (399) | T | ||||
| MLLex8/AF10 (E:589) | R2E | 268 (513) | T | ||||
| MLLex9/AF10(E:589) | R2E | 415 (660) | T | ||||
| MLLex5/AF10 (F:1931) | R2C | 493 | AML¶ | ||||
| t(10;14)(q24;q11) | HOX11 (10q24) | Karpas-299 | AML, ALL, CML¶ | ||||
| dupMLL (11q23) | MLL (11q23) | MLLex5/MLLex2 | R2F | 184 | ALL¶ | ||
| MLL (11q23) | MLLex6/MLLex2 | R2F | 258 | AML, ALL | 15, 20, 21 | ||
| MLLex7/MLLex2 | R2F | 145 (390) | AML | 21 | |||
| MLLex8/MLLex2 | R2F | 259 (504) | AML | 21 | |||
| MLLex9/MLLex2 | R2F | 406 (651) | AML | 21 | |||
| t(11;17)(q23;q21) | PLZF (11q23) | PLZF/RAR (A:1365) | R8A | 315 | APL | 58, 59 | |
| RARA (17q21) | PLZF/RAR(B:1452) | R8A | 402 | APL | 59 | ||
| t(11;17)(q23;q21) | MLL (11q23) | MLLex5/AF17 | R2B | 281 | AML | 11 | |
| AF17 (17q21) | |||||||
| t(11;19)(q23;p13.1) | MLL (11q23) | MLLex6/ELL | R1E | 330 | T | ||
| ELL (19p13.1) | MLLex7/ELL | R1E | 217 (462) | AML | 9, 60, 61 | ||
| MLLex8/ELL | R1E | 301 (576) | T | ||||
| MLLex9/ELL | R1E | 448 (723) | T | ||||
| MLLex6/ELL-ins120 | R1E | 450 | T | ||||
| MLLex7/ELL-ins120 | R1E | 337 (582) | AML | 60 | |||
| MLLex8/ELL-ins120 | R1E | 451 (696) | AML | 60 | |||
| MLLex9/ELL-ins120 | R1E | 598 (845) | T | ||||
| t(11;19)(q23;p13.3) | MLL(11q23) | MLLex6/ENL (A:177) | R5B | 186 | ALL | 14, 17 | |
| ENL (19p13.3) | MLLex7/ENL (A:177) | R5B | 73 (318) | KOCL-44§ | ALL | 14, 17 | |
| MLLex8/ENL(A:177) | R5B | 187 (432) | KOCL-44§ | ALL | 14, 17 | ||
| MLLex9/ENL (A:177) | R5B | 334 (579) | T | ||||
| t(12;21)(p13;q22) | TEL(12p13) | TEL/AML1 | R3C | 293 | ALL | 62 | |
| AML1 (21q22) | TEL/AML1 | R3C | 332 | ALL | 62-64 | ||
| t(15;17)(q21;q22) | PML (15q21) | PMLex3/RARα ex2 | R8C | 393 | NB4 | APL | 65-67 |
| RAR (17q21) | S-form (=BCR3) | ||||||
| PMLex3/RARα ex2 | R8C | 338 | NB4 | APL | 66 | ||
| S-form splice variant | |||||||
| PMLex6/RARα ex2 | R8B | 427 | NB4 | APL | 66, 67 | ||
| L-form (=BCR1) | |||||||
| PMLa ex3 Δex5 + 6/ | R8C | 464 | NB4 | APL | 67 | ||
| RARα ex2, | |||||||
| L-form splice variant | |||||||
| PML ex6-(+/−)ins- | R8B | +/−427 | APL | 66 | |||
| RAR ex2 | |||||||
| V-form (=BCR2) | |||||||
| inv(16)(p13q22) | CBF (16q22) | CBF/MYH11 (A) | R1A | 270 | ME-1§ | AML | 68 |
| MYH11 (16p13) | CBF/MYH11 (B) | R1B | 483 | AML | 68 | ||
| CBF/MYH11 (C) | R1B | 663 | AML | 68 | |||
| CBF/MYH11 (D) | R1A | 337 | AML | 68 | |||
| CBF/MYH11 (E) | R1A | 544 | AML | 68 | |||
| CBF/MYH11 (F) | R1B | 174 | AML | 68 | |||
| CBF/MYH11 (G) | R1B | 241 | AML | 68 | |||
| CBF/MYH11 (H) | R1B | 348 | AML | 68 | |||
| t(16;21)(p11;q22) | TLS (16p11) | TLS/ERG (a) | R4C | 318 | UTP-L12§ | AML | 69 |
| ERG (21q22) | TLS/ERG (b) | R4C | 274 | UTP-L12§ | AML | 69 | |
| TLS/ERG (c) | R4C | 239 | UTP-L12§ | AML, ALL | 69 | ||
| TLS/ERG(d) | R4C | 344 | ALL¶ | ||||
| TLS/ERG (e) | R4C | 413 | ALL¶ | ||||
| t(17;19)(q22;p13) | E2A(19p13) | E2Aex13/HLFex4 (I) | R3B | 390 | HAL-01 | ALL | 70-72 |
| HLF (17q22) | E2Aex13insHLFex4 (I) | R3B | 417 | ALL | 72 | ||
| E2Aex12/HLFex4 (II) | R3B | 207 | ALL | 72 |
Abbreviations: No, number; t, translocation; inv, inversion; p, short chromosome arm; q, long chromosome arm; ex, exon; ins, insertion; T, theoretically possible translocation variant; AML, acute myelogenous leukemia; ALL, acute lymphoblastic leukemia; CML (-BC), chronic myeloid leukemia (in blast crisis); MDS, myelodysplastic syndrome; APL, acute promyelocytic leukemia; AUL, acute undifferentiated leukemia; CMML, chronic myelomonocytic leukemia; ALCL, anaplastic large cell lymphoma; AMMOL, acute myelomonocytic leukemia.
*Letters and numbers in parentheses after the fusion-gene indicates alternative breakpoints and/or splice variants.
†R1 through R8 indicate multiplex reaction number; suffixes A through F indicate split-out reaction.
§Cell line not available for testing described as positive: ME-1 in reference 73, Karpas-45 in reference 7, Kasumi-1 in reference 74, SKH1 in reference 43, UTP-L12 in reference 69, KOCL-44 in reference 14.
‡Numbers in parentheses indicate the size of the co-amplified PCR fragment resulting from the MLL exon 5 primer.
¶Novel variants detected in this study.