Specific cDNA Primers
| Name* . | Sequence 5′ to 3′ . |
|---|---|
| ABL:797L12 | GCTGCCATTGAT |
| AF10:1150L12 | ACCTGAGCTGTG |
| AF10:2392L12 | CCACTGCCTCTC |
| AF10:750L13 | GTAGCCACAGTAT |
| AF17:1965L11 | GACACCGGAAG |
| AF1P:445L12 | ATGGGAGCTCAG |
| AF1Q:804L12 | AGGGCTTTTGAG |
| AF4:1664L12 | TTCCTTGCTGAG |
| AF6:355L12 | CCGATCATCTTT |
| AF9:1536L12 | CTGCCATCACTT |
| AF9:1910L12 | GCATCCAGTTGT |
| AFX1:70L12 | AAGTGCCAACAG |
| ALL1:417L13 | TTTGGTCTCTGAT |
| AML1A:1921L12 | CGGTAGCATTTC |
| AML1MDSEVI:2459L12 | CCCATCCATAAC |
| AML1MDSEVI:4905L12† | TAAGGCTGCTCT |
| CAN:2896L12 | TTGGCTGGTACT |
| E2A:1960L11 | GCTTCGCTCAG |
| ELL:456L12 | CCAGCCTTGATG |
| ENL:137L12 | TCTCCACGAAGT |
| ERG:1000L12 | TGTAGGCGTAGC |
| ETO:350L12 | GTGCGAACTCTT |
| HLF:1181L12 | GGCCTCATACTT |
| HOX11:897L11† | TGCTGCCTCTC |
| MLF1:320L12 | TGGTCTGGAAAG |
| MYH11:1552L12 | CTGCTGGGTGAG |
| MYH11:2271L12 | AGCTGCTTGATG |
| NPMALK:714L12 | CAGCGAACAATG |
| PBX1:526L11‡ | CCCTCCAGAAG |
| PDGFR:2451L12 | CTGCAGGAAGGT |
| RARA:700L12 | CGGTCGTTTCTC |
| TAL1:263L11 | CCGTGCCTCTA |
| Name* . | Sequence 5′ to 3′ . |
|---|---|
| ABL:797L12 | GCTGCCATTGAT |
| AF10:1150L12 | ACCTGAGCTGTG |
| AF10:2392L12 | CCACTGCCTCTC |
| AF10:750L13 | GTAGCCACAGTAT |
| AF17:1965L11 | GACACCGGAAG |
| AF1P:445L12 | ATGGGAGCTCAG |
| AF1Q:804L12 | AGGGCTTTTGAG |
| AF4:1664L12 | TTCCTTGCTGAG |
| AF6:355L12 | CCGATCATCTTT |
| AF9:1536L12 | CTGCCATCACTT |
| AF9:1910L12 | GCATCCAGTTGT |
| AFX1:70L12 | AAGTGCCAACAG |
| ALL1:417L13 | TTTGGTCTCTGAT |
| AML1A:1921L12 | CGGTAGCATTTC |
| AML1MDSEVI:2459L12 | CCCATCCATAAC |
| AML1MDSEVI:4905L12† | TAAGGCTGCTCT |
| CAN:2896L12 | TTGGCTGGTACT |
| E2A:1960L11 | GCTTCGCTCAG |
| ELL:456L12 | CCAGCCTTGATG |
| ENL:137L12 | TCTCCACGAAGT |
| ERG:1000L12 | TGTAGGCGTAGC |
| ETO:350L12 | GTGCGAACTCTT |
| HLF:1181L12 | GGCCTCATACTT |
| HOX11:897L11† | TGCTGCCTCTC |
| MLF1:320L12 | TGGTCTGGAAAG |
| MYH11:1552L12 | CTGCTGGGTGAG |
| MYH11:2271L12 | AGCTGCTTGATG |
| NPMALK:714L12 | CAGCGAACAATG |
| PBX1:526L11‡ | CCCTCCAGAAG |
| PDGFR:2451L12 | CTGCAGGAAGGT |
| RARA:700L12 | CGGTCGTTTCTC |
| TAL1:263L11 | CCGTGCCTCTA |
*The primer-names contain the name of the gene (or fusion-gene) used to construct the primer, the 5′ nucleotide number where the primer starts, U or L denotes forward or backward, and the length of the primer. Underlining indicates the gene where the primer is located.
Primers used for detection of HOX11 and EVI1expression. The use of these primers is not discussed further in this presentation.
PBX = PML.