Table 1. Primers Specific for Rh30 Genes... | American Society of Hematology

Table 1.

Primers Specific for Rh30 Genes

Category	Sequence (5′-3′)^*	Location	Position^-151
(I) Exon assay
5′-UTs^-152	ATCGCTCCCTCAAGCCCTCAAGTA	5′UT	−86-−63
Ex-1a	CTTTTGATCCTCTAAGGAAGCGTCATA	Ex 1	99-123
Ex-2s	GGCCAAGATCTGACCGTGATGGCG	Ex 2	151-174
Ex-2a	CTGAACAGTGTGATGACCACCTTC	Ex 2	312-335
Ex-4s	attgcagACAGACTACCACATGAAC	In 3/Ex 4
In-4a	tctgagccattctgctcagcccaa	In 4
In-4s	gacgtgacttccccatctaactct	In 4
Ex-5a	GCTGATCTTCCT(C)TTGGGGGTGAGC	Ex 5	775-798
Ex-6a	ACTTATGTGCACAGTGCGGTGTTGG	Ex 6	802-826
In-6a	tgtctagtttcttacCGGCAGG	In 6/Ex 6
Ex-7s	TGTTGTAACCGAGTGCTGGGGATT	Ex 7	943-966
In-7a	agtgcacacgccccagctaagga	In 7
In-8s	cttgagattaaaaatcctgtgctc	In 8
Ex-9a	CCAGAAAACTTGGTCATCAAAATATTT	Ex 9	1198-1224
Ex-10s	AGTTTCCTCATTTGGCTGTTGGATTT	Ex 10	1226-1250
3′-UTa(D)	GTATTCTACAGTGCATAATAAATGGTG	3′UT, D	1432-1458
3′-UTa(CE)	CTGTCTCTGACCTTGTTTCATTATAC	3′UT, CE	1363-1388
(II) Amplification of exon 7
In-6s	tttgaggtgagccttagtgcccat	In 6
Ex-7a	gtgacccacATGCCATTGCCGTTC	In 7/Ex 7
In-7a	Same as in (I)
(III) RT-PCR
5′-UTs	CCTGCACAGAGACGGACACA	5′UT	−33-−22
Ex-5a	TGGCCAGAACATCCACAAGAAGAG	Ex 5	663-640
Ex-4s	CCAAAATAGGCTGCGAACACGTAGA	Ex 4	515-539
Ex-9s	GGTTTGCTCCTAAATCTCAAAATATGG	Ex 9	1153-1179
3′-UTa(D)	Same as in (I)
3′-UTa(CE)	Same as in (I)

Category	Sequence (5′-3′)^*	Location	Position^-151
(I) Exon assay
5′-UTs^-152	ATCGCTCCCTCAAGCCCTCAAGTA	5′UT	−86-−63
Ex-1a	CTTTTGATCCTCTAAGGAAGCGTCATA	Ex 1	99-123
Ex-2s	GGCCAAGATCTGACCGTGATGGCG	Ex 2	151-174
Ex-2a	CTGAACAGTGTGATGACCACCTTC	Ex 2	312-335
Ex-4s	attgcagACAGACTACCACATGAAC	In 3/Ex 4
In-4a	tctgagccattctgctcagcccaa	In 4
In-4s	gacgtgacttccccatctaactct	In 4
Ex-5a	GCTGATCTTCCT(C)TTGGGGGTGAGC	Ex 5	775-798
Ex-6a	ACTTATGTGCACAGTGCGGTGTTGG	Ex 6	802-826
In-6a	tgtctagtttcttacCGGCAGG	In 6/Ex 6
Ex-7s	TGTTGTAACCGAGTGCTGGGGATT	Ex 7	943-966
In-7a	agtgcacacgccccagctaagga	In 7
In-8s	cttgagattaaaaatcctgtgctc	In 8
Ex-9a	CCAGAAAACTTGGTCATCAAAATATTT	Ex 9	1198-1224
Ex-10s	AGTTTCCTCATTTGGCTGTTGGATTT	Ex 10	1226-1250
3′-UTa(D)	GTATTCTACAGTGCATAATAAATGGTG	3′UT, D	1432-1458
3′-UTa(CE)	CTGTCTCTGACCTTGTTTCATTATAC	3′UT, CE	1363-1388
(II) Amplification of exon 7
In-6s	tttgaggtgagccttagtgcccat	In 6
Ex-7a	gtgacccacATGCCATTGCCGTTC	In 7/Ex 7
In-7a	Same as in (I)
(III) RT-PCR
5′-UTs	CCTGCACAGAGACGGACACA	5′UT	−33-−22
Ex-5a	TGGCCAGAACATCCACAAGAAGAG	Ex 5	663-640
Ex-4s	CCAAAATAGGCTGCGAACACGTAGA	Ex 4	515-539
Ex-9s	GGTTTGCTCCTAAATCTCAAAATATGG	Ex 9	1153-1179
3′-UTa(D)	Same as in (I)
3′-UTa(CE)	Same as in (I)

*Intronic sequences are denoted by lowercase letters and others by uppercase letters.

Nucleotide positions of exon primers are accounted from the first base of initiation codon ATG.

Sense (s) and antisense (a) primers to 3′-UT, exon (Ex), and intron (In) are denoted.