Influence of IL-7 on SCID Mouse FTOC
| . | Total Cells/Lobe (×10−4) . | Dendritic Cells . | |
|---|---|---|---|
| % . | Absolute No. (×10−2) . | ||
| C | 3.5 ± 0.9 | 2.6 | 9.2 ± 1.2 |
| IL-7 | 10.3 ± 1.6* | 8.6 | 88.7 ± 7.2† |
| . | Total Cells/Lobe (×10−4) . | Dendritic Cells . | |
|---|---|---|---|
| % . | Absolute No. (×10−2) . | ||
| C | 3.5 ± 0.9 | 2.6 | 9.2 ± 1.2 |
| IL-7 | 10.3 ± 1.6* | 8.6 | 88.7 ± 7.2† |
Thymic lobes from fetal day 14 SCID mice were organ-cultured in the presence of IL-7. After 12 days of culture, cells from thymic lobes were counted, cytospun onto slides, and stained with anti–MHC class II MoAbs. Percentages of MHC class II+ cells exhibiting DC morphology were obtained scoring 5,000 cells in 5 to 7 cytospin preparations. The results represent means ± SEM of three independent experiments.
Statistical significance differences between control and IL-7–treated FTOC: *P ≤ .01; †P ≤ .001.