Effect of Fas Triggering on CFU-C Growth From CD34+ BM Cells of CML Patients Sensitive or Resistant to IFN-α
| . | Sensitive n = 7 . | Resistant n = 3 . | ||
|---|---|---|---|---|
| Control . | Fas Triggering . | Control . | Fas Triggering . | |
| Untreated | 100 | 63.6 ± 5 | 100 | 97 ± 1 |
| IFN-α 20 U/mL | 91.3 ± 4 | 51.7 ± 9 | 101 ± 6 | 88 ± 7 |
| IFN-α 100 U/mL | 82.8 ± 7 | 48.7 ± 13 | 106 ± 2 | 88 ± 6 |
| IFN-α 1,000 U/mL | 65.4 ± 5 | 25.8 ± 11 | 99.3 ± 6 | 80.3 ± 3 |
| . | Sensitive n = 7 . | Resistant n = 3 . | ||
|---|---|---|---|---|
| Control . | Fas Triggering . | Control . | Fas Triggering . | |
| Untreated | 100 | 63.6 ± 5 | 100 | 97 ± 1 |
| IFN-α 20 U/mL | 91.3 ± 4 | 51.7 ± 9 | 101 ± 6 | 88 ± 7 |
| IFN-α 100 U/mL | 82.8 ± 7 | 48.7 ± 13 | 106 ± 2 | 88 ± 6 |
| IFN-α 1,000 U/mL | 65.4 ± 5 | 25.8 ± 11 | 99.3 ± 6 | 80.3 ± 3 |
Values (mean ± SEM) represent colony formation as a percentage of untreated CML progenitors. For untreated cells, the mean number of CFU-C (erythroid and myeloid)/103 CD34+ plated was 105 ± 9 for responders and 112 ± 33 for nonresponders. Fas was triggered by the agonist MoAb CH11 used at the concentration of 1 μg/mL. Statistical analysis (paired t-test) showed P< .05 only in the responder group, for control versus IFN-α 100 and 1,000 U/mL; control versus Fas triggering; control + any IFN-α concentration versus control + any IFN-α concentration + Fas triggering.