Effect of Fas Triggering on Apoptosis of CD34+ BM Cells From CML Patients Sensitive or Resistant to IFN-α
| . | Sensitive n = 5 . | Resistant n = 3 . | ||
|---|---|---|---|---|
| Control . | Fas Triggering . | Control . | Fas Triggering . | |
| Untreated | 7 ± 1 | 14.3 ± 3 | 8.6 ± 1 | 7.8 ± 1 |
| IFN-α 20 U/mL | 9.3 ± 1 | 13.7 ± 4 | 6.5 ± 1 | 8.1 ± 1 |
| IFN-α 100 U/mL | 11 ± 3 | 17.7 ± 5 | 6 ± 1 | 7 ± 1 |
| IFN-α 1,000 U/mL | 16.6 ± 3 | 20.2 ± 1 | 6.8 ± 2 | 11.8 ± 2 |
| . | Sensitive n = 5 . | Resistant n = 3 . | ||
|---|---|---|---|---|
| Control . | Fas Triggering . | Control . | Fas Triggering . | |
| Untreated | 7 ± 1 | 14.3 ± 3 | 8.6 ± 1 | 7.8 ± 1 |
| IFN-α 20 U/mL | 9.3 ± 1 | 13.7 ± 4 | 6.5 ± 1 | 8.1 ± 1 |
| IFN-α 100 U/mL | 11 ± 3 | 17.7 ± 5 | 6 ± 1 | 7 ± 1 |
| IFN-α 1,000 U/mL | 16.6 ± 3 | 20.2 ± 1 | 6.8 ± 2 | 11.8 ± 2 |
Values (mean ± SEM) represent percentage of apoptotic CD34+ BM CML cells evaluated by the Tdt assay. The Fas agonist MoAb CH11 was used at the concentration of 1 μg/mL. Each experiment was performed in triplicate. Statistical analysis (pairedt-test) showed P < .05 only in the responder group, for control versus IFN-α 100 and 1,000 U/mL; control versus Fas triggering; control + any IFN-α concentration versus control + any IFN-α concentration + Fas triggering.