Ex Vivo Culture, Infusion, and Engraftment Data on All Animals
| Animal . | Culture Conditions* . | Total Cell (no./kg × 106) . | CFU (no./kg × 105) . | Day ANC >500/μL¶ . | ||||
|---|---|---|---|---|---|---|---|---|
| Postcolumn-151 . | End Transduction-152 . | End Expansion-153 . | Postcolumn-151 . | End Transduction-152 . | End Expansion-153 . | |||
| RQ813 | IL-3/IL-6/SCF:10 | 6.4 | 11.0 | 114 | 3.6 | 2.0 | 7.4 | 5 |
| RQ923 | IL-3/IL-6/SCF:10 | 5.2 | 10.4 | 104 | 3.9 | 2.5 | 8.3 | 5 |
| RQ1271 | IL-3/IL-6/SCF:14 | 7.1 | 8.0 | 186 | 3.9 | 3.0 | 63.7 | 6 |
| RQ1094 | IL-3/IL-6/SCF:14 | 4.0 | 11.0 | 130 | 3.3 | 2.6 | 27.9 | 6 |
| L161 | IL-3/IL-6/SCF/FLT:14 | 5.8 | 11.7 | 131 | 3.3 | 4.1 | 29.5 | 7 |
| J039 | IL-3/IL-6/SCF/FLT:14 | 1.3 | 3.1 | 41.1 | 0.9 | 1.5 | 4.5 | 17 |
| RC402 | IL-3/IL-6/SCF/FLT/STR:14 | 5.4 | 12.8 | 76.1 | 4.1 | 3.9 | 11.5 | 7 |
| 94E048 | IL-3/IL-6/SCF/FLT/STR:14 | 4.6 | 5.3 | 27.0 | 1.4 | 0.7 | 1.9 | 19 |
| Animal . | Culture Conditions* . | Total Cell (no./kg × 106) . | CFU (no./kg × 105) . | Day ANC >500/μL¶ . | ||||
|---|---|---|---|---|---|---|---|---|
| Postcolumn-151 . | End Transduction-152 . | End Expansion-153 . | Postcolumn-151 . | End Transduction-152 . | End Expansion-153 . | |||
| RQ813 | IL-3/IL-6/SCF:10 | 6.4 | 11.0 | 114 | 3.6 | 2.0 | 7.4 | 5 |
| RQ923 | IL-3/IL-6/SCF:10 | 5.2 | 10.4 | 104 | 3.9 | 2.5 | 8.3 | 5 |
| RQ1271 | IL-3/IL-6/SCF:14 | 7.1 | 8.0 | 186 | 3.9 | 3.0 | 63.7 | 6 |
| RQ1094 | IL-3/IL-6/SCF:14 | 4.0 | 11.0 | 130 | 3.3 | 2.6 | 27.9 | 6 |
| L161 | IL-3/IL-6/SCF/FLT:14 | 5.8 | 11.7 | 131 | 3.3 | 4.1 | 29.5 | 7 |
| J039 | IL-3/IL-6/SCF/FLT:14 | 1.3 | 3.1 | 41.1 | 0.9 | 1.5 | 4.5 | 17 |
| RC402 | IL-3/IL-6/SCF/FLT/STR:14 | 5.4 | 12.8 | 76.1 | 4.1 | 3.9 | 11.5 | 7 |
| 94E048 | IL-3/IL-6/SCF/FLT/STR:14 | 4.6 | 5.3 | 27.0 | 1.4 | 0.7 | 1.9 | 19 |
*IL-3 (20 ng/mL), IL-6 (50 ng/mL), SCF (100 ng/mL), FLT (100 ng/mL), and STR coculture on autologous marrow stromal cell layer. For each animal, these culture conditions were used for both the transduction and expansion periods. The total length of culture for the expanded fraction is shown in days.
The number of total cells or CFU present after CD34 column immunoabsorption, expressed per kilogram of recipient animal.
The number of total cells or CFU present in the nonexpanded fraction at the end of the 96-hour transduction period (note only 50% of the starting postcolumn CD34-enriched cells were used in this fraction) expressed per kilogram of recipient.
The number of total cells or CFU present in the expanded fraction at the end of the 10- to 14-day expansion period (note only 50% of the starting postcolumn CD34-enriched cells were used in this fraction) expressed per kilogram of recipient.
¶Day 0 defined as the day of reinfusion.