Oligonucletide Primers for the Amplification and Detection of Cytokines
| Molecule . | Sequence Reference . | Primer . | Sequence (5′ to 3′) . | Position . | Predicted Size . |
|---|---|---|---|---|---|
| SCF | Zsebo27 | Forward | TCCTGTGACTGATATGT | 108-125 | 419 bp |
| Backward | GACCTAATGTTGAAGAGA | 509-526 | |||
| G-CSF | Tsuchiya28 | Forward | GCTGTGGCAAAAGTGCACT | 121-138 | 417 bp |
| Backward | ATCTGCTGCCAGATGGTG | 520-537 | |||
| M-CSF | Harrington29 | Forward | ACCCAGCTGCCCGTATAG | 168-185 | 311 bp |
| Backward | AAAGCGCATGGTCTCATC | 461-478 | |||
| GM-CSF | Miyatake30 | Forward | GAGAGAAAGGCTAAGGTCCT | 5-24 | 500 bp |
| Backward | AAAAGCAGCAGTCTGAGAAG | 485-504 | |||
| β-Actin | Tokunaga31 | Forward | TCAGAAGGACTCCTATGTGG | 224-243 | 500 bp |
| Backward | TCTCTTTGATGTCACGCACG | 704-723 |
| Molecule . | Sequence Reference . | Primer . | Sequence (5′ to 3′) . | Position . | Predicted Size . |
|---|---|---|---|---|---|
| SCF | Zsebo27 | Forward | TCCTGTGACTGATATGT | 108-125 | 419 bp |
| Backward | GACCTAATGTTGAAGAGA | 509-526 | |||
| G-CSF | Tsuchiya28 | Forward | GCTGTGGCAAAAGTGCACT | 121-138 | 417 bp |
| Backward | ATCTGCTGCCAGATGGTG | 520-537 | |||
| M-CSF | Harrington29 | Forward | ACCCAGCTGCCCGTATAG | 168-185 | 311 bp |
| Backward | AAAGCGCATGGTCTCATC | 461-478 | |||
| GM-CSF | Miyatake30 | Forward | GAGAGAAAGGCTAAGGTCCT | 5-24 | 500 bp |
| Backward | AAAAGCAGCAGTCTGAGAAG | 485-504 | |||
| β-Actin | Tokunaga31 | Forward | TCAGAAGGACTCCTATGTGG | 224-243 | 500 bp |
| Backward | TCTCTTTGATGTCACGCACG | 704-723 |