Flow Cytometry Analysis in Children With PAP After Therapy
| Patient No. . | AML Therapy . | βc . | GM-CSFR α . | IL-3R α . | Material . |
|---|---|---|---|---|---|
| 1 | ChTx + CBT | 95% | 93% | 25% | BM |
| 98% | 92% | ND | BAL | ||
| 13-150 | ChTx; TBI + BMT | 99% | 87% | 10% | BM |
| 2 | Chtx | 82% | 80% | 13% | BM |
| 3 | ChTx; TBI + BMT | 92% | 92% | 25% | BM (th) |
| Patient No. . | AML Therapy . | βc . | GM-CSFR α . | IL-3R α . | Material . |
|---|---|---|---|---|---|
| 1 | ChTx + CBT | 95% | 93% | 25% | BM |
| 98% | 92% | ND | BAL | ||
| 13-150 | ChTx; TBI + BMT | 99% | 87% | 10% | BM |
| 2 | Chtx | 82% | 80% | 13% | BM |
| 3 | ChTx; TBI + BMT | 92% | 92% | 25% | BM (th) |
Flow cytometry analysis of the GM-CSF/IL-3/IL-5 receptors common β chain (βc), GM-CSF receptor α chain (GM-CSFR α), and IL-3 receptor α chain (IL-3Rα) in bone marrow aspirate (BM) or broncheo alveolar lavage fluid (BAL) from 3 children with AML and PAP symptoms. Data are expressed as the percentage of cells in the myeloid gate.
Abbreviations: BMT, allogeneic bone marrow transplantation; CBT, allogeneic cord blood transplantation; Chtx, chemotherapy; TBI, total body irradiation; th, thawed.
Patient no. 1 relapsed with his AML 3 months after allogeneic cord blood transplantation, graft failure, and autologous reconstitution (see case report).