Effects of Anti–VLA-4 Antibody on Adherence of BM CD34+ Cells to Stromal Cell Monolayer
| Addition of Antibody . | Recovery of Nonadherent CD34+ Cells (×103) . | ||
|---|---|---|---|
| Total Cells . | Cells in S + G2/M . | Cells in G0/G1 . | |
| None | 80 ± 7.1 | 7.3 ± 0.70 | 73 ± 7.8 |
| Control antibody | 78 ± 5.9 | 6.6 ± 0.87 | 72 ± 5.2 |
| Anti–VLA-4 antibody | 100 ± 2.9 | 11 ± 0.95 | 88 ± 2.8 |
| Addition of Antibody . | Recovery of Nonadherent CD34+ Cells (×103) . | ||
|---|---|---|---|
| Total Cells . | Cells in S + G2/M . | Cells in G0/G1 . | |
| None | 80 ± 7.1 | 7.3 ± 0.70 | 73 ± 7.8 |
| Control antibody | 78 ± 5.9 | 6.6 ± 0.87 | 72 ± 5.2 |
| Anti–VLA-4 antibody | 100 ± 2.9 | 11 ± 0.95 | 88 ± 2.8 |
A total of 1 × 105 steady-state BM cells, 12% ± 1.2% of which were in S + G2/M phase of the cell cycle, were incubated on a monolayer of MS-5 cells in the presence or absence of control antibody or anti–VLA-4 MoAb. After a 1-hour incubation nonadherent cells were obtained by gentle agitation and subjected to cell-cycle analysis. The results of 3 experiments are summarized.
P < .001 by multiple t-test.