Table 3.

Inhibition of Spontaneous Apoptosis in B-CLL Cells by CD23 Ligation and Reversal by NOS Inhibitor

Incubation With: % Annexin V+ Cells
B-CLL No. 1 B-CLL No. 2 B-CLL No. 3
Medium  50.7  46.5  34.9  
Anti-CD23  34.5  27.8 13.3  
Anti-CD23 + L-NMMA  48.1  42.1  28.2 
Incubation With: % Annexin V+ Cells
B-CLL No. 1 B-CLL No. 2 B-CLL No. 3
Medium  50.7  46.5  34.9  
Anti-CD23  34.5  27.8 13.3  
Anti-CD23 + L-NMMA  48.1  42.1  28.2 

B-CLL cells from three different patients were resuspended at 1 × 106/mL in RPMI-1640 medium supplemented with 5% FCS and incubated for 48 hours with or without 10 μg/mL of anti-CD23 MoAb (MoAb135) and in the presence or absence of L-NAME (1 mmol/L). At the end of the culture, the percentages of Annexin V+ cells were assessed by FACS analysis on the total population (regions 1 and 2, as determined in Figs 8 and 9).

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