Inhibition of Spontaneous Apoptosis in B-CLL Cells by CD23 Ligation and Reversal by NOS Inhibitor
| Incubation With: . | % Annexin V+ Cells . | ||
|---|---|---|---|
| B-CLL No. 1 . | B-CLL No. 2 . | B-CLL No. 3 . | |
| Medium | 50.7 | 46.5 | 34.9 |
| Anti-CD23 | 34.5 | 27.8 | 13.3 |
| Anti-CD23 + L-NMMA | 48.1 | 42.1 | 28.2 |
| Incubation With: . | % Annexin V+ Cells . | ||
|---|---|---|---|
| B-CLL No. 1 . | B-CLL No. 2 . | B-CLL No. 3 . | |
| Medium | 50.7 | 46.5 | 34.9 |
| Anti-CD23 | 34.5 | 27.8 | 13.3 |
| Anti-CD23 + L-NMMA | 48.1 | 42.1 | 28.2 |
B-CLL cells from three different patients were resuspended at 1 × 106/mL in RPMI-1640 medium supplemented with 5% FCS and incubated for 48 hours with or without 10 μg/mL of anti-CD23 MoAb (MoAb135) and in the presence or absence of L-NAME (1 mmol/L). At the end of the culture, the percentages of Annexin V+ cells were assessed by FACS analysis on the total population (regions 1 and 2, as determined in Figs 8 and 9).