Microinjection of Granzyme B Directly Into the Cytosol of Target Cell Resulted in Apoptosis
| U/cell . | fg/Cell . | Granzyme B . | Death/Total . | |
|---|---|---|---|---|
| nmol/L/Cell . | % Apoptosis . | |||
| 1 × 10−6 | 27 | 179.4 | 3.34 ± 0.51 | 19/559-150 |
| 2 × 10−6 | 54 | 358.9 | 28.41 ± 21.01 | 17/684-151 |
| 3 × 10−6 | 81 | 538.3 | 41.32 ± 3.08 | 233/485-151 |
| 4 × 10−6 | 108 | 717.8 | 59.09 | 166/286 |
| Inactive granzyme B | ||||
| 3 × 10−6 | 81 | 538.3 | 0.00 ± 0.00 | 0/587-150 |
| U/cell . | fg/Cell . | Granzyme B . | Death/Total . | |
|---|---|---|---|---|
| nmol/L/Cell . | % Apoptosis . | |||
| 1 × 10−6 | 27 | 179.4 | 3.34 ± 0.51 | 19/559-150 |
| 2 × 10−6 | 54 | 358.9 | 28.41 ± 21.01 | 17/684-151 |
| 3 × 10−6 | 81 | 538.3 | 41.32 ± 3.08 | 233/485-151 |
| 4 × 10−6 | 108 | 717.8 | 59.09 | 166/286 |
| Inactive granzyme B | ||||
| 3 × 10−6 | 81 | 538.3 | 0.00 ± 0.00 | 0/587-150 |
Granzyme B, but not inactivated granzyme B, induced cell death by direct injection into the cytoplasm of MCF-7 cells in 2 hours. Percent apoptosis represents number of dead cells divided by the total injected cells and then subtracted percent death of control cells, which were injected only with 0.3% Texas Red and 0.15 mol/L NaCl.
Represents total number of cells from two experiments.
Represents total number of cells from three experiments.