Peritoneal Cell Surface Marker Distribution Within Forward/Side Scatter Regions
| Surface Markers . | % Positive Cells . | |||||
|---|---|---|---|---|---|---|
| Region 1 . | Region 2 . | |||||
| 0 h . | 16 h . | 96 h . | 0 h . | 16 h . | 96 h . | |
| c-Fms | 72 (2) | 35 (3) | 92 (1) | 5 (1) | 14 (1) | 72 (5) |
| F4/80 | 74 (5) | 22 (8) | 8 (3) | 5 (1) | 9 (3) | 19 (5) |
| MUM-4 | 37 (1) | ND | 2 (1) | 0 | ND | 0 |
| Class II | 12 (1) | 35 (4) | 29 (1) | 57 (14) | 8 (2) | 10 (2) |
| T and B | 3 (1) | 6 (1) | 1 | 76 (5) | 26 (2) | 44 (3) |
| Mac-1 | 83 (6) | 92 (1) | 95 (1) | 19 (8) | 90 (1) | 79 (5) |
| Surface Markers . | % Positive Cells . | |||||
|---|---|---|---|---|---|---|
| Region 1 . | Region 2 . | |||||
| 0 h . | 16 h . | 96 h . | 0 h . | 16 h . | 96 h . | |
| c-Fms | 72 (2) | 35 (3) | 92 (1) | 5 (1) | 14 (1) | 72 (5) |
| F4/80 | 74 (5) | 22 (8) | 8 (3) | 5 (1) | 9 (3) | 19 (5) |
| MUM-4 | 37 (1) | ND | 2 (1) | 0 | ND | 0 |
| Class II | 12 (1) | 35 (4) | 29 (1) | 57 (14) | 8 (2) | 10 (2) |
| T and B | 3 (1) | 6 (1) | 1 | 76 (5) | 26 (2) | 44 (3) |
| Mac-1 | 83 (6) | 92 (1) | 95 (1) | 19 (8) | 90 (1) | 79 (5) |
Six surface markers (see the Materials and Methods) were used to help define regions from the forward/side scatter profiles represented in Fig 3 (see text). Peritoneal cells were taken before (0 hours) and after (16 and 96 hours) TM injection. Region 1: “macrophage” region at t = 0; Region 2: “lymphocyte” region at t = 0, “lymphocyte/macrophage” region after 16 hours. Data are presented as a percentage of the cells within the region expressing the particular marker. Data are the mean (SEM) from 3 experiments (8 mice). A partial kinetic analysis was also performed another 8 times.
Abbreviation: ND, not done.