IgM Production in JD38 Cell Lines
| Cells . | Intracellular IgM (lysate) . | Culture Supernatant IgM . |
|---|---|---|
| JD38 | 2,993.0 ± 105.2-150 | 1,249.7 ± 164.6 |
| LXSN | 2,654.5 ± 278.8 | 1,441.5 ± 77.6 |
| TIMP-1(20) | 1,107.1 ± 4.5 | 239.9 ± 20.3 |
| TIMP-1(24) | 968.4 ± 46.0 | 273.7 ± 21.5 |
| Cells . | Intracellular IgM (lysate) . | Culture Supernatant IgM . |
|---|---|---|
| JD38 | 2,993.0 ± 105.2-150 | 1,249.7 ± 164.6 |
| LXSN | 2,654.5 ± 278.8 | 1,441.5 ± 77.6 |
| TIMP-1(20) | 1,107.1 ± 4.5 | 239.9 ± 20.3 |
| TIMP-1(24) | 968.4 ± 46.0 | 273.7 ± 21.5 |
TIMP-1 over-expressing JD38 cell clones 20 and 24 as well as vector control and parental JD38 cells were analyzed by ELISA for intracellular (lysate column) and secreted IgM levels in culture supernatants. A significant decrease in total IgM production is shown by TIMP-1 cell clones compared with controls. TIMP-1–JD38 cells also show downregulation of cell surface IgM (Fig 5B). Data represent triplicate determinations ± SD of three experiments.
IgM concentration (ng/mL) ± SD.