Table 3.

Yield and Phenotype of DC Generated After 7-Day Culture in the Presence of GM-CSF and IL-4

Mitomycin C Treatment Donor 1Donor 2 Donor 3
+ − +− + −
Total yield (×105/well)  6.5 6.7  10  8  9  9  
% Recovery  33  34  50 40  45  45  
DC yield (% total cells)  46 54  59  61  70  66 
 % Positive Recovered Cells  
HLA-DR  68  58  48  39  78 76  
CD14  9  7  5  1  13  3  
CD40  55 42  36  51  47  69  
CD1a  30  55  53  64 49  70 
Mitomycin C Treatment Donor 1Donor 2 Donor 3
+ − +− + −
Total yield (×105/well)  6.5 6.7  10  8  9  9  
% Recovery  33  34  50 40  45  45  
DC yield (% total cells)  46 54  59  61  70  66 
 % Positive Recovered Cells  
HLA-DR  68  58  48  39  78 76  
CD14  9  7  5  1  13  3  
CD40  55 42  36  51  47  69  
CD1a  30  55  53  64 49  70 

Freshly isolated cells were treated with or without mitomycin C then cultured. The total yield was calculated based on a starting population of 2 × 106 cells/well. The DC yield was calculated by flow cytometry, based on forward and side scatter properties.10 

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