HLA Supermotifs and Putative Peptides of Adhesion Molecules Which Are the Candidate of Immunodominant mHa
| HLA Superfamily4-151 . | Supermotif* . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| B Pocket . | F Pocket . | ||||||||||
| A3-like | AILMVST | RK | |||||||||
| B44-like | DE | AILMVFWY | |||||||||
| Candidate of mHa | Putative Peptide Sequences Position of Peptide | Supermotif | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ||
| CD49b (GPIa) | R | V | Y | L | F | T | I | K | E/K | A3-like motif | |
| CD31 (PECAM-I) exon 12 | N | D | D | V | G/R | N | H | A | M | B44-like motif | |
| CD62L (LECAM-I) | M | D | C | T | H | P/S | L | G | N | F | B44-like motif |
| HLA Superfamily4-151 . | Supermotif* . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| B Pocket . | F Pocket . | ||||||||||
| A3-like | AILMVST | RK | |||||||||
| B44-like | DE | AILMVFWY | |||||||||
| Candidate of mHa | Putative Peptide Sequences Position of Peptide | Supermotif | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ||
| CD49b (GPIa) | R | V | Y | L | F | T | I | K | E/K | A3-like motif | |
| CD31 (PECAM-I) exon 12 | N | D | D | V | G/R | N | H | A | M | B44-like motif | |
| CD62L (LECAM-I) | M | D | C | T | H | P/S | L | G | N | F | B44-like motif |
*The amino acid polymorphisms within B pocket (which is normally occupied by the side-chain of the position 2 residue of bound peptides) and F pocket (which is normally occupied by the C-terminal residue of bound peptides) of the HLA class I binding groove were shared between HLA antigens of the same superfamily. The putative peptide from CD49b fit into the A3-like supermotif of bound peptides carrying some hydrophobic residues at position 2 (V) and positively charged residues in the C-terminal (K). The polymorphic amino acid (E/K) of CD49b is the C-terminal residue. In CD31, the putative peptide that fit into the B44-like supermotif was found in exon 12 (position 2; D and C-terminal; M), which is the cytoplasmic domain. In CD62L, the putative peptide that fit into the B44-like supermotif (position 2; D and C-terminal; F) was also found. Amino acids are represented by one character. Underlines show the polymorphic amino acids in these adhesion molecules.
HLA superfamily based on similarities of their peptide-binding motifs, such as HLA-A3-like (A3, A11, A31, A33 and A*6801), B44-like (B37, B41, B44, B45, B47, B49, B50, B60 and B61).29