The Percentage of NBT-Positive and NB4-R1 Ribozyme-Transfected Cells
| Ribozyme . | Hygromycin (μg/mL) . | ATRA (10−6 mol/L) . | % NBT Positive . |
|---|---|---|---|
| NB4-S1 | 0 | + | 85% |
| NB4-R1 | 0 | + | 1% |
| APL 1.1 | 65 | − | 1% |
| APL 1.1 | 65 | + | 1% |
| APL 1.1 | 195 | − | 1% |
| APL 1.1 | 195 | + | 1% |
| APL 5.0 | 65 | − | 1% |
| APL 5.0 | 65 | + | 1% |
| APL 5.0 | 195 | − | 1% |
| APL 5.0 | 195 | + | 1% |
| Ribozyme . | Hygromycin (μg/mL) . | ATRA (10−6 mol/L) . | % NBT Positive . |
|---|---|---|---|
| NB4-S1 | 0 | + | 85% |
| NB4-R1 | 0 | + | 1% |
| APL 1.1 | 65 | − | 1% |
| APL 1.1 | 65 | + | 1% |
| APL 1.1 | 195 | − | 1% |
| APL 1.1 | 195 | + | 1% |
| APL 5.0 | 65 | − | 1% |
| APL 5.0 | 65 | + | 1% |
| APL 5.0 | 195 | − | 1% |
| APL 5.0 | 195 | + | 1% |
NB4-R1 cells transfected with APL 1.1 or APL 5.0 were cultured as indicated and the NBT assay was performed 5 days after the addition of ATRA (or not) and an increase (or not) in the hygromycin dosage. NB4-S1 cells were used as a positive and parental NB4-R1 cells were a negative control, respectively. This assay was conducted on cells harvested on day 5 from the treatment arms set up for the MTT assays.