Effect of IGF-1 Alone or in Combination With GM-CSF on IL-10–Induced Suppression of Autonomous BFU-E Growth From PV Cells
| Culture Conditions . | BFU-E ± SD/Dish . |
|---|---|
| Control (no cytokines) | 96.0 ± 8.2 |
| + 10 ng/mL IL-10 | 12.7 ± 3.1 |
| + 10 ng/mL IL-10 + 1 ng/mL IGF-1 | 10.3 ± 0.6 |
| + 10 ng/mL IL-10 + 10 ng/mL IGF-1 | 14.0 ± 2.7 |
| + 10 ng/mL IL-10 + 100 ng/mL IGF-1 | 13.7 ± 1.5 |
| + 10 ng/mL IL-10 + 100 U/mL GM-CSF | 83.7 ± 4.2 |
| + 10 ng/mL IL-10 + 100 U/mL GM-CSF + 10 ng/mL IGF-1 | 92.7 ± 24.6 |
| Culture Conditions . | BFU-E ± SD/Dish . |
|---|---|
| Control (no cytokines) | 96.0 ± 8.2 |
| + 10 ng/mL IL-10 | 12.7 ± 3.1 |
| + 10 ng/mL IL-10 + 1 ng/mL IGF-1 | 10.3 ± 0.6 |
| + 10 ng/mL IL-10 + 10 ng/mL IGF-1 | 14.0 ± 2.7 |
| + 10 ng/mL IL-10 + 100 ng/mL IGF-1 | 13.7 ± 1.5 |
| + 10 ng/mL IL-10 + 100 U/mL GM-CSF | 83.7 ± 4.2 |
| + 10 ng/mL IL-10 + 100 U/mL GM-CSF + 10 ng/mL IGF-1 | 92.7 ± 24.6 |
A total of 105 PBMNC/dish from patient SA were cultured in methylcellulose in the presence or absence of exogenous cytokines as indicated in the Table. Colony growth was assessed after 14 days. Results represent mean values ± SD from triplicates.