Percentage of Apoptotic Cells as a Function of Drug Treatment and Time
| . | Vehicle . | DEX . | 8Cl-AD . | 8Cl-AD + ADA . | 8Cl-cAMP . | 8Cl-cAMP + ADA . | ADA . |
|---|---|---|---|---|---|---|---|
| 24 hours | 5.26 ± 0.22 | 39.81 ± 1.67 | 30.23 ± 0.34 | 6.25 ± 0.15 | 12.93 ± 1.26 | 10.9 ± 1.09 | 5.02 ± 0.48 |
| 48 hours | 6.77 ± 0.24 | 17.38 ± 1.89 | 30.12 ± 0.37 | 9.45 ± 0.66 | 28.12 ± 1.02 | 16.2 ± 0.03 | 7.74 ± 0.36 |
| . | Vehicle . | DEX . | 8Cl-AD . | 8Cl-AD + ADA . | 8Cl-cAMP . | 8Cl-cAMP + ADA . | ADA . |
|---|---|---|---|---|---|---|---|
| 24 hours | 5.26 ± 0.22 | 39.81 ± 1.67 | 30.23 ± 0.34 | 6.25 ± 0.15 | 12.93 ± 1.26 | 10.9 ± 1.09 | 5.02 ± 0.48 |
| 48 hours | 6.77 ± 0.24 | 17.38 ± 1.89 | 30.12 ± 0.37 | 9.45 ± 0.66 | 28.12 ± 1.02 | 16.2 ± 0.03 | 7.74 ± 0.36 |
MM.1S cells were treated with either 8Cl-cAMP (5 μmol/L) or 8Cl-AD (5 μmol/L) for the indicated times and then assayed using a flow cytometric assay for apoptosis (see Materials and Methods). Dexamethasone (1 μmol/L) was included as a positive control. Where indicated, cells were treated in the presence of 1 U/mL ADA. Data are presented as the average of three independent assays with standard deviation.