Table 1. Summary of Single-Cell Analysis... | American Society of Hematology

Table 1.

Summary of Single-Cell Analysis of Two Cases for V Gene Rearrangements

Patient No.	Experiment No.	Cells Positive for V Rearrangements After PCR With Different Forward Primers				PCR Products		Rearrangements
Patient No.	Experiment No.	V_κ	V_HFRI	V_HFRII	Clone- Specific	Total	Sequenced	Repeated	Unique
1	1a	ND	13/17	—^-150	ND	13	13	13 V_H1
	1b	ND	7/14	—^-150	7/14	7	7	7 V_H1
2	2′LN	0/13	0/13	—^-150	3/13	3	3	3 V_H1
	2"LN	0/7	—^-150	1/7	3/7	3	3	3 V_H1
	2"spleen	0/10	—^-150	1/10	2/10	2	2	2 V_H1
Control	1a^-151	ND	6/8 T cells	—^-150	ND	8	8		5 V_H3,3 V_H4
	1b	ND	0/8 T cells	—^-150	0/8 T cells
		ND	0/10 buffer	—^-150	0/10 buffer
Control	2′LN	0/4 T cells	0/4 T cells	—^-150	0/4 T cells
	2"LN	0/6 T cells	—^-150	0/6 T cells	0/6 T cells
	2"spleen	0/5 T cells	—^-150	0/5 T cells	0/5 T cells

Patient No.	Experiment No.	Cells Positive for V Rearrangements After PCR With Different Forward Primers				PCR Products		Rearrangements
Patient No.	Experiment No.	V_κ	V_HFRI	V_HFRII	Clone- Specific	Total	Sequenced	Repeated	Unique
1	1a	ND	13/17	—^-150	ND	13	13	13 V_H1
	1b	ND	7/14	—^-150	7/14	7	7	7 V_H1
2	2′LN	0/13	0/13	—^-150	3/13	3	3	3 V_H1
	2"LN	0/7	—^-150	1/7	3/7	3	3	3 V_H1
	2"spleen	0/10	—^-150	1/10	2/10	2	2	2 V_H1
Control	1a^-151	ND	6/8 T cells	—^-150	ND	8	8		5 V_H3,3 V_H4
	1b	ND	0/8 T cells	—^-150	0/8 T cells
		ND	0/10 buffer	—^-150	0/10 buffer
Control	2′LN	0/4 T cells	0/4 T cells	—^-150	0/4 T cells
	2"LN	0/6 T cells	—^-150	0/6 T cells	0/6 T cells
	2"spleen	0/5 T cells	—^-150	0/5 T cells	0/5 T cells

H-RS cells were analyzed together with B and T cells coded in a blinded fashion from two patients with HD. The number of PCR products obtained and sequenced from H-RS and T cells for the first and the second experiment is indicated. Clonally independent V_Hgene rearrangements of various V_H gene families were obtained from control B cells (not shown). Repeated rearrangements indicate clonally related rearrangements; unique rearrangements indicate rearrangements amplified once and not related to any other rearrangement. The sequences obtained from H-RS cells of both patients were deposited in the Genbank under the accession numbers AJ007389 and AJ0007390.

Abbreviations: LN, lymph node; ND, not determined.

F0-150

PCR was performed either with V_HFRI or with V_HFRII primers.

F0-151

In this experiment, a B-cell–specific antibody was eventually used for the “T-cell staining.”

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