Flow Cytometric Assessment of GM-CSF and IL-3 Receptor Component Expression on Peripheral Blood Cells
| Patient No. . | Myelomonocytic Cells* (% of total WBC) . | Expression of Specific Receptor Components (% relative to myelomonocytic cell content) . | ||
|---|---|---|---|---|
| βc . | GM-CSF α-chain . | IL-3 α-chain . | ||
| Acquired PAP | ||||
| 1 | 54 | 94 | 30 | 102 |
| 2 | 47 | 66 | 79 | 72 |
| 7 | 27 | 93 | 107 | 96 |
| 9 | 57 | 67 | 91 | 88 |
| 10 | 50 | 66 | 166 | 130 |
| 11 | 58 | 95 | 138 | 112 |
| Mean (SD) | 49 (11) | 80 (15) | 102 (47) | 100 (20) |
| Congenital PAP | ||||
| 12 | 70 | 76 | 80 | Not tested |
| 13 | 54 | 122 | 139 | 93 |
| 15 | 70 | 90 | 134 | 127 |
| Mean (SD) | 65 (9) | 96 (24) | 118 (33) | 110 (17) |
| Normal controls | ||||
| A | 45 | 93 | 69 | 93 |
| B | 70 | 86 | 101 | 84 |
| C | 47 | 64 | 87 | 77 |
| Mean (SD) | 54 (14) | 81 (15) | 86 (16) | 85 (8) |
| Patient No. . | Myelomonocytic Cells* (% of total WBC) . | Expression of Specific Receptor Components (% relative to myelomonocytic cell content) . | ||
|---|---|---|---|---|
| βc . | GM-CSF α-chain . | IL-3 α-chain . | ||
| Acquired PAP | ||||
| 1 | 54 | 94 | 30 | 102 |
| 2 | 47 | 66 | 79 | 72 |
| 7 | 27 | 93 | 107 | 96 |
| 9 | 57 | 67 | 91 | 88 |
| 10 | 50 | 66 | 166 | 130 |
| 11 | 58 | 95 | 138 | 112 |
| Mean (SD) | 49 (11) | 80 (15) | 102 (47) | 100 (20) |
| Congenital PAP | ||||
| 12 | 70 | 76 | 80 | Not tested |
| 13 | 54 | 122 | 139 | 93 |
| 15 | 70 | 90 | 134 | 127 |
| Mean (SD) | 65 (9) | 96 (24) | 118 (33) | 110 (17) |
| Normal controls | ||||
| A | 45 | 93 | 69 | 93 |
| B | 70 | 86 | 101 | 84 |
| C | 47 | 64 | 87 | 77 |
| Mean (SD) | 54 (14) | 81 (15) | 86 (16) | 85 (8) |
*Myelomonocytic cells defined as those expressing CD15 by flow cytometry.