Expression of Fas in MM Cell Lines
| . | Fas-Negative Cells . | Fas-Positive Cells . | MFI . |
|---|---|---|---|
| U-266-1970 | |||
| FCS + IL-6 IgG FITC | 91% | 6% | 4.5 |
| FCS + IL-6 | 9% | 87% | 24.8 |
| +1,000 U/mL IFN-γ | 1% | 94% | 48.0 |
| +1,000 U/mL IFN-α | 2% | 92% | 60.6 |
| U-266-1984 | |||
| FCS IgG FITC | 86% | 3% | 2.6 |
| FCS | 2% | 84% | 17.9 |
| +1,000 U/mL IFN-γ | 2% | 78% | 24.6 |
| +1,000 U/mL IFN-α | 1% | 85% | 27.2 |
| U-1958 | |||
| FCS + IL-6 IgG FITC | 82% | 8% | 3.3 |
| FCS + IL-6 | 35% | 55% | 14.7 |
| +1,000 U/mL IFN-γ | 33% | 51% | 17.6 |
| +1,000 U/mL IFN-α | 28% | 59% | 16.0 |
| . | Fas-Negative Cells . | Fas-Positive Cells . | MFI . |
|---|---|---|---|
| U-266-1970 | |||
| FCS + IL-6 IgG FITC | 91% | 6% | 4.5 |
| FCS + IL-6 | 9% | 87% | 24.8 |
| +1,000 U/mL IFN-γ | 1% | 94% | 48.0 |
| +1,000 U/mL IFN-α | 2% | 92% | 60.6 |
| U-266-1984 | |||
| FCS IgG FITC | 86% | 3% | 2.6 |
| FCS | 2% | 84% | 17.9 |
| +1,000 U/mL IFN-γ | 2% | 78% | 24.6 |
| +1,000 U/mL IFN-α | 1% | 85% | 27.2 |
| U-1958 | |||
| FCS + IL-6 IgG FITC | 82% | 8% | 3.3 |
| FCS + IL-6 | 35% | 55% | 14.7 |
| +1,000 U/mL IFN-γ | 33% | 51% | 17.6 |
| +1,000 U/mL IFN-α | 28% | 59% | 16.0 |
Cells were harvested and analyzed by flow cytometry after 96 hours of incubation in the presence of IL-6 and with or without the addition of IFN-γ (1,000 U/mL) or IFN-α (1,000 U/mL).