Oligonucleotides Primers Used for RT-PCR Analyses
| Gene . | Amplicon Size (bp) . | Sense . | Antisense . | PCR Cycles . | Positive Control . |
|---|---|---|---|---|---|
| β-major globin | 447 | 5′ ATGGTGCACCTGACTGATGCTG 3′ | 5′ GGTTTAGTGGTACTTGTGAGCC 3′ | 30 | BM |
| εy-globin | 419 | 5′ AACCCTCATCAATGGCCTGTGG 3′ | 5′ TCAGTGGTACTTGTGGGACAGC 3′ | 30 | FL |
| c-kit | 458 | 5′ GCTCATAAATGGCATGCTCCAGTGT 3′ | 5′ GAAGTTGCGTCGGGTCTATGTAAAC 3′ | 36 | BM |
| GATA-1 | 263 | 5′ ACTCGTCATACCACTAAGGT 3′ | 5′ AGTGTCTGTAGGCCTCAGCT 3′ | 40 | BM |
| p45 | 288 | 5′ TCAGCAGAACAGGAACAGGT 3′ | 5′ GCTTTGACACTGGTATAGCT 3′ | 36 | BM |
| SCL | 277 | 5′ TAGCCTTAGCCAGCCGCTCG 3′ | 5′ GCGGAGGATCTCATTCTTGC 3′ | 43 | BM |
| EKLF | 360 | 5′ TCGCCGGAGACGCAGGCT 3′ | 5′ CCCAGTCCTTGTGCAGGA 3′ | 43 | BM |
| GATA-2 | 336 | 5′ TGCAACACACCACCCGATACC 3′ | 5′ CAATTTGGACAACAGGTGCCC 3′ | 43 | BM |
| c-myb | 522 | 5′ GAGCTTGTCCAGAAATATGGTCCTAAG 3′ | 5′ GGCTGCCGCAGCCGGCTGAGGGAC 3′ | 36 | BM |
| Nrf2 | 223 | 5′ CGAAAAGGAAAGACAAGAGC 3′ | 5′ TGGGAATGTCTCTGCCAAAA 3′ | 36 | BM |
| GATA-3 | 246 | 5′ TCTCACTCTCGAGGCAGCATGA 3′ | 5′ GGTACCATCTCGCCGCCACAG 3′ | 40 | BW5147 |
| ALAS-E | 581 | 5′ GTCCTGTGGAGGAATTGTGT 3′ | 5′ GTTTTCCATCATCTGAGGGC 3′ | 32 | BM |
| ALAD | 450 | 5′ AAGGAGCCTGAGAGAGTGGGAGCA 3′ | 5′ AGCTGCAGAGCCCTGTTCATCCTT 3′ | 34 | BM |
| PBGD | 431 | 5′ ACAACAGATCCTATTACAGCTTTT 3′ | 5′ AAGGTTTCCAGGGTCTTTCCAATA 3′ | 34 | BM |
| FC | 195 | 5′ TGTGGAGCTGAGAACAT 3′ | 5′ TCACAGCTGTTGGCTGG 3′ | 34 | BM |
| HPRT | 249 | 5′ GCTGGTGAAAAGGACCTCT 3′ | 5′ CACAGGACTAGAACACCTGC 3′ | 36 |
| Gene . | Amplicon Size (bp) . | Sense . | Antisense . | PCR Cycles . | Positive Control . |
|---|---|---|---|---|---|
| β-major globin | 447 | 5′ ATGGTGCACCTGACTGATGCTG 3′ | 5′ GGTTTAGTGGTACTTGTGAGCC 3′ | 30 | BM |
| εy-globin | 419 | 5′ AACCCTCATCAATGGCCTGTGG 3′ | 5′ TCAGTGGTACTTGTGGGACAGC 3′ | 30 | FL |
| c-kit | 458 | 5′ GCTCATAAATGGCATGCTCCAGTGT 3′ | 5′ GAAGTTGCGTCGGGTCTATGTAAAC 3′ | 36 | BM |
| GATA-1 | 263 | 5′ ACTCGTCATACCACTAAGGT 3′ | 5′ AGTGTCTGTAGGCCTCAGCT 3′ | 40 | BM |
| p45 | 288 | 5′ TCAGCAGAACAGGAACAGGT 3′ | 5′ GCTTTGACACTGGTATAGCT 3′ | 36 | BM |
| SCL | 277 | 5′ TAGCCTTAGCCAGCCGCTCG 3′ | 5′ GCGGAGGATCTCATTCTTGC 3′ | 43 | BM |
| EKLF | 360 | 5′ TCGCCGGAGACGCAGGCT 3′ | 5′ CCCAGTCCTTGTGCAGGA 3′ | 43 | BM |
| GATA-2 | 336 | 5′ TGCAACACACCACCCGATACC 3′ | 5′ CAATTTGGACAACAGGTGCCC 3′ | 43 | BM |
| c-myb | 522 | 5′ GAGCTTGTCCAGAAATATGGTCCTAAG 3′ | 5′ GGCTGCCGCAGCCGGCTGAGGGAC 3′ | 36 | BM |
| Nrf2 | 223 | 5′ CGAAAAGGAAAGACAAGAGC 3′ | 5′ TGGGAATGTCTCTGCCAAAA 3′ | 36 | BM |
| GATA-3 | 246 | 5′ TCTCACTCTCGAGGCAGCATGA 3′ | 5′ GGTACCATCTCGCCGCCACAG 3′ | 40 | BW5147 |
| ALAS-E | 581 | 5′ GTCCTGTGGAGGAATTGTGT 3′ | 5′ GTTTTCCATCATCTGAGGGC 3′ | 32 | BM |
| ALAD | 450 | 5′ AAGGAGCCTGAGAGAGTGGGAGCA 3′ | 5′ AGCTGCAGAGCCCTGTTCATCCTT 3′ | 34 | BM |
| PBGD | 431 | 5′ ACAACAGATCCTATTACAGCTTTT 3′ | 5′ AAGGTTTCCAGGGTCTTTCCAATA 3′ | 34 | BM |
| FC | 195 | 5′ TGTGGAGCTGAGAACAT 3′ | 5′ TCACAGCTGTTGGCTGG 3′ | 34 | BM |
| HPRT | 249 | 5′ GCTGGTGAAAAGGACCTCT 3′ | 5′ CACAGGACTAGAACACCTGC 3′ | 36 |
Abbreviations: BM, bone marrow; FL, fetal liver.