Effect of Exogenous IL-10 on Cytokine Production by EBV-Infected PBL
| Experiment . | Day . | IL-2 pg/mL . | IL-6 pg/mL . | IL-10 pg/mL . | IFN-γ pg/mL . | TNF-α pg/mL . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control . | IL-10 . | Control . | IL-10 . | Control . | Control . | IL-10 . | Control . | IL-10 . | ||
| 1 | 3 | 80 | 0 | 983 | 0 | 218 | 1,603 | 1,113 | 61 | 27 |
| 2 | 3 | 40 | 12 | 329 | 0 | 829 | 2,156 | 629 | NT | NT |
| 3 | 3 | 60 | 0 | 818 | 0 | 631 | 2,838 | 1,176 | NT | NT |
| 4 | 1 | 45 | 26 | 2,000 | 537 | 147 | NT | NT | 740 | 237 |
| 2 | 295 | 66 | 1,077 | 188 | 277 | NT | NT | 214 | 35 | |
| 3 | 260 | 65 | 1,394 | 182 | 255 | 1,897 | 365 | 221 | 41 | |
| 5 | 1 | 44 | 16 | 1,005 | 995 | 165 | NT | NT | 772 | 130 |
| 2 | 71 | 13 | 1,390 | 565 | 290 | NT | NT | 91 | 37 | |
| 3 | 164 | 52 | 2,002 | 482 | 427 | 2,636 | 547 | 52 | 22 | |
| Experiment . | Day . | IL-2 pg/mL . | IL-6 pg/mL . | IL-10 pg/mL . | IFN-γ pg/mL . | TNF-α pg/mL . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control . | IL-10 . | Control . | IL-10 . | Control . | Control . | IL-10 . | Control . | IL-10 . | ||
| 1 | 3 | 80 | 0 | 983 | 0 | 218 | 1,603 | 1,113 | 61 | 27 |
| 2 | 3 | 40 | 12 | 329 | 0 | 829 | 2,156 | 629 | NT | NT |
| 3 | 3 | 60 | 0 | 818 | 0 | 631 | 2,838 | 1,176 | NT | NT |
| 4 | 1 | 45 | 26 | 2,000 | 537 | 147 | NT | NT | 740 | 237 |
| 2 | 295 | 66 | 1,077 | 188 | 277 | NT | NT | 214 | 35 | |
| 3 | 260 | 65 | 1,394 | 182 | 255 | 1,897 | 365 | 221 | 41 | |
| 5 | 1 | 44 | 16 | 1,005 | 995 | 165 | NT | NT | 772 | 130 |
| 2 | 71 | 13 | 1,390 | 565 | 290 | NT | NT | 91 | 37 | |
| 3 | 164 | 52 | 2,002 | 482 | 427 | 2,636 | 547 | 52 | 22 | |
PBL from EBV-seropositive individuals were exposed to the virus and cultured in medium control or medium containing 100 U/mL of hIL-10. The cell-free supernatants were collected and analyzed for cytokine content with cytokine specific ELISA.
Abbreviation: NT, not tested.