Colonies Generated From CD34+ Cell Subpopulations
| CD34+ Cell Source . | CFC-GM . | BFU-E . | ||||||
|---|---|---|---|---|---|---|---|---|
| MIP-1 R+ . | MIP-1 R− . | MIP-1 R+ . | MIP-1 R− . | |||||
| Colony No. . | % of Total . | Colony No. . | % of Total . | Colony No. . | % of Total . | Colony No. . | % of Total . | |
| NBM (n = 3) | 48.7 ± 3.2 | 65.3 ± 17.8 | 34.0 ± 9.6 | 34.7 ± 17.8 | 86.0 ± 31.5 | 56.2 ± 7.0 | 68.3 ± 38.0 | 43.8 ± 7.0 |
| CB (n = 4) | 43.5 ± 14.7 | 92.1 ± 5.5-151 | 18.0 ± 5.3 | 7.9 ± 5.5 | 118.3 ± 30.8 | 85.2 ± 5.0-151 | 130 ± 26.2 | 14.8 ± 5.0 |
| LP (n = 5) | 10.0 ± 2.0-150 | 63.6 ± 12.3 | 4.0 ± 1.0 | 36.4 ± 12.3 | 112 ± 29.7 | 51.1 ± 18.3 | 94.8 ± 40.0 | 48.9 ± 18.3 |
| CD34+ Cell Source . | CFC-GM . | BFU-E . | ||||||
|---|---|---|---|---|---|---|---|---|
| MIP-1 R+ . | MIP-1 R− . | MIP-1 R+ . | MIP-1 R− . | |||||
| Colony No. . | % of Total . | Colony No. . | % of Total . | Colony No. . | % of Total . | Colony No. . | % of Total . | |
| NBM (n = 3) | 48.7 ± 3.2 | 65.3 ± 17.8 | 34.0 ± 9.6 | 34.7 ± 17.8 | 86.0 ± 31.5 | 56.2 ± 7.0 | 68.3 ± 38.0 | 43.8 ± 7.0 |
| CB (n = 4) | 43.5 ± 14.7 | 92.1 ± 5.5-151 | 18.0 ± 5.3 | 7.9 ± 5.5 | 118.3 ± 30.8 | 85.2 ± 5.0-151 | 130 ± 26.2 | 14.8 ± 5.0 |
| LP (n = 5) | 10.0 ± 2.0-150 | 63.6 ± 12.3 | 4.0 ± 1.0 | 36.4 ± 12.3 | 112 ± 29.7 | 51.1 ± 18.3 | 94.8 ± 40.0 | 48.9 ± 18.3 |
CD34+ cells were sorted into MIP-1α receptor positive and negative fractions (as shown in Fig 1D) and plated in colony-forming cell assays. The data are presented as the number of colonies formed per 2,000 cells plated and expressed as mean ± SEM of n independent experiments with triplicate determinations.
Indicates significant difference (P ≤ .05) between CFC-GM generation of the two subfractions.
Indicates significant difference (P ≤ .05) in the percentage of total CFC-GM and BFU-E between the two subfractions.