Table 2. Cell Cycle Status of MIP-1–R... | American Society of Hematology

Table 2.

Cell Cycle Status of MIP-1–R⁺ and MIP-1–R⁻ Cells in Cytokine-Stimulated CD34⁺ Cells

Cell Population	G₀/G₁	S/G₂/M
Total	64.5 ± 4.6	35.5 ± 4.6
MIP-1α–R⁺	58.1 ± 4.6	41.9 ± 4.6
MIP-1α–R⁻	82.4 ± 0.6^*	17.6 ± 0.6^*

Cell Population	G₀/G₁	S/G₂/M
Total	64.5 ± 4.6	35.5 ± 4.6
MIP-1α–R⁺	58.1 ± 4.6	41.9 ± 4.6
MIP-1α–R⁻	82.4 ± 0.6^*	17.6 ± 0.6^*

LP CD34⁺ cells were incubated for 48 hours in IMDM/15% (vol/vol) FCS supplemented with IL-3 (100 ng/mL), IL-6 (1,000 U/mL), and SCF (50 ng/mL). Subsequently, cells were washed and double-labeled with biotinylated MIP-1α and propidium iodide to assess cell cycle status. DNA histograms were obtained for ungated, MIP-1α–R⁺ and MIP-1α–R⁻ cells as detailed in Materials and Methods and analyzed using Modfit LT software (Verity Software House, Maine). Data are percentages and shown as mean ± SEM of four independent experiments.

Denotes significant difference (P < .05) comparing the MIP-1α–R⁺ and MIP-1α–R⁻subpopulations.

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