Expression of MIP-1 Binding Chemokine Receptor Subtypes by CD34+ Cells
| Patient No. . | CCR-1 . | CCR-4 . | CCR-5 . | |||
|---|---|---|---|---|---|---|
| % Positive Cells . | MFI . | % Positive Cells . | MFI . | % Positive Cells . | MFI . | |
| 1 | 67.7 | 8.4 | 7.8 | 3.4 | 32.7 | 5.5 |
| 2 | 63.8 | 31.5 | 1.0 | 8.9 | 28.3 | 20.3 |
| Patient No. . | CCR-1 . | CCR-4 . | CCR-5 . | |||
|---|---|---|---|---|---|---|
| % Positive Cells . | MFI . | % Positive Cells . | MFI . | % Positive Cells . | MFI . | |
| 1 | 67.7 | 8.4 | 7.8 | 3.4 | 32.7 | 5.5 |
| 2 | 63.8 | 31.5 | 1.0 | 8.9 | 28.3 | 20.3 |
Highly purified (purity >90%) LP CD34+ cells were analyzed for the expression of chemokine receptor subtypes using single-color flow cytometry (FACS). Details of the staining protocol and FACS analysis are given in Material and Methods.