ES-Cell Contribution to TM Chimeric Mice
| . | GPI . | APC . |
|---|---|---|
| High-percentage chimeras (n = 5) | 33% (range, 26% to 40%) | 14 ng/minute (3)* |
| Low-percentage chimeras (n = 5) | 20% (range, 18% to 23%) | 17 ng/minute (3) |
| . | GPI . | APC . |
|---|---|---|
| High-percentage chimeras (n = 5) | 33% (range, 26% to 40%) | 14 ng/minute (3)* |
| Low-percentage chimeras (n = 5) | 20% (range, 18% to 23%) | 17 ng/minute (3) |
Lung lysates prepared from TM−/− chimeric mice were used to identify GPI by cellulose acetate gel electrophoresis and staining for GPI activity. The percentage of each isozyme was determined from digitized images of the cellulose acetate gels. Using the same lung tissue from each mouse the APC was determined.
Abbreviation: APC, PC cofactor activity.
The standard deviation for the APC measurement is shown in parentheses.